A method for tissue culture and rapid propagation of Xianwang jujube
A technique for tissue culture rapid propagation and presenting king jujube, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc. It can solve the problems of different tissue culture methods for jujube trees, large differences in breeding conditions, and difficulties in jujube tissue culture. , to achieve the effects of accelerating rapid popularization and development and utilization, increasing reproduction speed, and easy plant utilization
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0025] A method for tissue culture and rapid propagation of Xianwang jujube, comprising the following steps:
[0026] (1) Establishment of explants: Before budding in spring, cut off the 1-year-old jujube head branches, cut off the tender shoots and the aging parts of the base, and leave the semi-lignified stems, rinse with tap water for 10 minutes, and dissolve them at a mass concentration of Soak in 0.4% washing powder solution for 8 minutes, then rinse with tap water, then soak in 75% alcohol for 35 seconds, rinse with sterile water 3 times, drain the water, and disinfect with 1.0% sodium hypochlorite solution for 10 minutes , and then rinsed with sterile water 7 times, picked up the cleaned stems with tweezers, blotted the surface moisture with sterilized filter paper on the aseptic operating table, and cut 1.5-2.5cm budded stems as Explant;
[0027] (2) Start-up culture: Inoculate the sterilized explants into the sterilized start-up medium for culture. The composition of...
Embodiment 2
[0033] A method for tissue culture and rapid propagation of Xianwang jujube, comprising the following steps:
[0034] (1) Establishment of explants: Before budding in spring, cut off the 1-year-old jujube head branches, cut off the tender shoots and the aging parts of the base, and leave the semi-lignified stems, rinse with tap water for 10 minutes, and dissolve them at a mass concentration of Soak in 0.4% washing powder solution for 10 minutes, then rinse with tap water, then soak in 75% alcohol for 35 seconds, rinse with sterile water 4 times, drain the water, and disinfect with 1.0% sodium hypochlorite solution for 11 minutes , and then rinsed with sterile water 8 times, picked up the cleaned stems with tweezers, blotted the surface moisture with sterilized filter paper on the aseptic operating table, and cut 1.5-2.5cm budded stems as Explant;
[0035] (2) Start-up culture: Inoculate the sterilized explants into the sterilized start-up medium for culture. The composition o...
Embodiment 3
[0041] A method for tissue culture and rapid propagation of Xianwang jujube, comprising the following steps:
[0042] (1) Establishment of explants: Before budding in spring, cut off the 1-year-old jujube head branches, cut off the tender shoots and the aging parts of the base, and leave the semi-lignified stems, rinse with tap water for 10 minutes, and dissolve them at a mass concentration of Soak in 0.4% washing powder solution for 12 minutes, then rinse with tap water, then soak in 75% alcohol for 35 seconds, rinse with sterile water 4 times, drain the water, and disinfect with 1.0% sodium hypochlorite solution for 12 minutes , and then rinsed with sterile water 8 times, picked up the cleaned stems with tweezers, blotted the surface moisture with sterilized filter paper on the aseptic operating table, and cut 1.5-2.5cm budded stems as Explant;
[0043] (2) Start-up culture: Inoculate the sterilized explants into the sterilized start-up medium for culture. The composition o...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com