A kind of hollyhock stem and leaf polysaccharide and its preparation method and application

A technology of hollyhocks, stems and leaves is applied in the field of preparation of polysaccharides of stems and leaves of hollyhock, can solve the problems of waste, lack of effective utilization of stem and leaf resources, waste of resources, etc., and achieves easy enrichment, promotion of spleen lymphocyte proliferation, and improvement of production efficiency. Effect
CN106008729BActive Publication Date: 2018-05-25NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
Publication Date
2018-05-25

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Abstract

The invention discloses an abelmoschus manihot stem and leaf polysaccharide and a preparation method and an application thereof. The preparation method comprises the steps: extracting, carrying out ethanol precipitation, carrying out chromatography purification with a macroporous adsorption resin column, separating with a hollow cellulose membrane, carrying out chromatography separation with a DEAE cellulose resin column, collecting target fractions, carrying out desalting treatment, freeze-drying, and thus obtaining the abelmoschus manihot stem and leaf polysaccharide. The abelmoschus manihot stem and leaf polysaccharide has the weight-average molecular weight of 13821 Da and is composed of rhamnose, mannose, galactose, galacturonic acid, glucose and arabinose, and the molar ratio of rhamnose to mannose to galactose to galacturonic acid to glucose to arabinose is 1 to 0.53 to 0.12 to 0.05 to 0.16 to 0.03; the abelmoschus manihot stem and leaf polysaccharide has a good immune enhancing activity, and can be used for development of immune enhancing drugs and health food. Stem and leaf waste resources produced in a production process of abelmoschus manihot medicinal materials are used as raw materials, the polysaccharide substance having the immune activity is separated, and a basis is laid for cyclic utilization of the traditional Chinese medicine resources.
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Description

technical field

[0001] The invention relates to a traditional Chinese medicine extract, in particular to a preparation method and application of the stem and leaf polysaccharide of marshmallow with immune activity. Background technique

[0002] Hollyhock (Abelmoschus manihot (L.) Medic.) is a one-year to perennial herb of the Malvaceae genus Abelmoschus. It was first recorded in "Jiayou Materia Medica" written by Zhang Yuxi in the Song Dynasty. All have records, and included in the past editions of "The Pharmacopoeia of the People's Republic of China". In the past dynasties, herbal medicines mostly used their flowers as medicine, followed by their seeds, and their roots, stems and leaves were rarely used. Hollyhock flower is currently one of the main raw materials of traditional Chinese medicine for the treatment of kidney disease. The market demand is huge, but the stem and leaf resources after harvesting the flowers are largely discarded due to lack of effective utilizati...

Claims

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