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Method for improving viability of DC-CIK cell preparation
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A DC-CIK, cell preparation technology, applied in cell dissociation methods, animal cells, vertebrate cells, etc., can solve problems such as affecting the quality of preparations, reducing the acquisition rate of live cells, and lysis of live cells, and achieves the removal of dead cells, Conducive to clinical reinfusion and increasing cell viability
Inactive Publication Date: 2016-10-12
SINOBIOWAY CELL THERAPY CO LTD
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Problems solved by technology
The predecessors used a centrifugal force of 500g in the preparation. Such a high centrifugal force can separate living cells from dead cells, and can also cause lysis of living cells, thereby reducing the acquisition rate of living cells and affecting the quality of the preparation.
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Embodiment 1
[0017] A method for improving the viability of DC-CIK cell preparations, comprising the steps of:
[0018] S1. Centrifuging the cultured and expanded T cells for the first time to obtain centrifuged cells;
[0019] S2. After washing the primary centrifuged cells with physiological saline, perform a second centrifugation to obtain secondary centrifuged cells;
[0020] S3. After washing the secondary centrifuged cells with physiological saline, perform a third centrifugation to obtain a DC-CIK cell preparation.
Embodiment 2
[0022] A method for improving the viability of DC-CIK cell preparations, comprising the steps of:
[0023] S1. Centrifuge the cultured and expanded T cells for the first time to obtain centrifuged cells, the centrifugal force is 300g, and the centrifugation time is 15min;
[0024] S2. After washing the primary centrifuged cells with normal saline, perform a second centrifugation to obtain secondary centrifuged cells, the centrifugal force is 300g, and the centrifugation time is 10 min;
[0025] S3. After washing the secondary centrifuged cells with physiological saline, perform a third centrifugation to obtain a DC-CIK cell preparation, the centrifugal force is 300 g, and the centrifugation time is 10 min.
Embodiment 3
[0027] A method for improving the viability of DC-CIK cell preparations, comprising the steps of:
[0028] S1. Centrifuge the cultured and expanded T cells for the first time to obtain centrifuged cells, the centrifugal force is 500g, and the centrifugation time is 5min;
[0029] S2. After washing the primary centrifuged cells with normal saline, perform a second centrifugation to obtain secondary centrifuged cells, the centrifugal force is 500g, and the centrifugation time is 2min;
[0030] S3. After washing the secondary centrifuged cells with physiological saline, perform a third centrifugation to obtain a DC-CIK cell preparation, the centrifugal force is 500 g, and the centrifugation time is 2 min.
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Abstract
The invention discloses a method for improving the viability of a DC-CIK cell preparation. The method includes the following steps that primary centrifugation is conducted on cultured and proliferated T cells to obtain primary centrifugal cells; after the primary centrifugal cells are washed with physiological saline, secondary centrifugation is performed to obtain secondary centrifugal cells; after the secondary centrifugal cells are washed with physiological saline, tertiarycentrifugation is performed to obtain the DC-CIK cell preparation. The method can effectively remove dead cells, increase the cell viability of the obtained cell preparation, the obtained cell viability can be above 90%, and the cell preparation is beneficial to clinical retransfusion.
Description
technical field [0001] The invention relates to the technical field of the viability of DC-CIK cell preparations, in particular to a method for improving the viability of DC-CIK cell preparations. Background technique [0002] Autoimmune T cell technology is to isolate T cells from the peripheral blood of patients, culture, expand, and prepare them in vitro, and infuse them back into the patient's body to induce the body's own immune response. At present, the entire culture cycle of DC-CIK technology is about 14 days. With the increase of culture time and individual differences, the cell viability will decrease after culture in the later stage, and it may be lower than the standard (greater than 80%). When a large amount of reinfusion occurs, the increase of dead cells will increase some other risks clinically. The predecessors used a centrifugal force of 500g in the preparation. Such a high centrifugal force can separate living cells from dead cells, and can also cause lys...
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