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Neomycin sulfate ultraviolet spectrophotometer detection method

A neomycin sulfate and spectrophotometer technology, applied in the direction of color/spectral characteristic measurement, measuring device, analysis material, etc., can solve the problems of increasing the risk of the operation process, and achieve easy operation, reduced impact, safe and convenient use Effect

Inactive Publication Date: 2016-10-26
ANHUI HUAAO BIOTECH
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Problems solved by technology

The current chemical methods for detecting neomycin are commonly used as derivative reagents such as 2,4-dinitrofluorobenzene (DNFB), o-phthalaldehyde (OPA), ninhydrin-pyridine, acetylacetone-formaldehyde, etc. Most of them are toxic reagents and organic solvents, which increase the danger of the operation process and bring adverse effects to the body

Method used

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Embodiment Construction

[0021] Due to the long reaction time or the use of more organic solvents and other problems in methods such as thin layer chromatography, high performance liquid chromatography, and spectrophotometry. The purpose of this example is to provide a general and fast method for detecting the content of neomycin sulfate in medicines, which meets the needs of neomycin sulfate production control and preparation detection, and replaces the time-consuming pharmacopoeia method—microbiological assay.

[0022] A neomycin sulfate ultraviolet spectrophotometer detection method comprises the following steps:

[0023] (1) Accurately weigh 0.5 g of neomycin sulfate standard substance, and prepare a 20 mg / mL standard curve preparation solution;

[0024] (2) Precisely measure 1mL, 2mL, 3mL, 4mL, 5mL, 7.5mL of the standard curve preparation solution into a 25mL volumetric flask, add 2-5mL alkaline copper tartrate test solution, and dilute to the mark with pure water to prepare Neomycin sulfate sta...

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Abstract

The invention discloses a neomycin sulfate ultraviolet spectrophotometer detection method. According to the method, the conditions for forming the neomycin sulfate and Cu<2+> complex are studied, wherein a reaction is performed for 10-30 min at a room temperature in a seignette salt alkaline medium to generate the blue complex, wherein the maximum absorption is at the wavelength of 631 nm. According to the present invention, with the application of the method to determine the recovery rate and the content of the neomycin sulfate raw material and finished product soluble powder, the results show that the method has good universality and quickness, is not interfered by other coexisting substances in the preparation, provides the rapid neomycin sulfate content detection method for the medicine industry, and further has characteristics of strong specificity, good precision, and simple operation.

Description

technical field [0001] The invention relates to a neomycin sulfate ultraviolet spectrophotometer detection method. Background technique [0002] Neomycin sulfate soluble powder is an aminoglycoside antibiotic preparation that is easily soluble in water, stable in nature and widely used. It is widely used in veterinary clinics to treat Staphylococcus, Shigella, Escherichia coli, Proteus and other infections in livestock and poultry. caused by gastrointestinal infections. At present, the Chinese Pharmacopoeia 2010 edition adopts the microbiological assay method to determine the content. The operation is complex, the measurement cycle is long, the reagents are expensive, and there are many influencing factors. However, with the development of new technologies and the improvement of equipment automation, the accuracy of this method has been further improved. However, it is not suitable for semi-finished products in the production process. Quality control, this method will grad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N1/28G01N1/38G01N1/34
CPCG01N21/33G01N1/28G01N1/34G01N1/38G01N2001/386
Inventor 孟庆娟杨晓龙张广志胡广伟
Owner ANHUI HUAAO BIOTECH
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