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Optical analyzer

A technology of optical analysis and light source, applied in the field of optical analyzers, can solve problems such as optical system configuration and shutter mechanism complexity, and achieve low-cost effects

Inactive Publication Date: 2016-11-02
SHIMADZU SEISAKUSHO CO LTD
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, even according to this configuration, the configuration of the optical system, the shutter mechanism, etc. are complicated

Method used

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Embodiment Construction

[0034] Hereinafter, an absorbance / fluorescence detector according to an embodiment of the present invention will be described with reference to the drawings.

[0035] figure 1 Is a schematic configuration diagram of the absorbance / fluorescence detector of this embodiment, Figure 2A , Figure 2B with Figure 2C It is the operation time chart of the absorbance / fluorescence detector of this embodiment. The sample cell 2 in which the target sample solution flows is connected to, for example, a column outlet of the LC, and a sample solution containing a plurality of components temporally separated in the column flows in the sample cell 2 at a substantially constant flow rate.

[0036] figure 1 Among them, the light projection unit 1 including a single LED as its light source is driven by the driving current supplied by the LED driving unit 5 to emit light. The light emitted from the light projection unit 1 is projected into the sample cell 2 as measurement light and excitation light. ...

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Abstract

Disclosed is an optical analyzer. Light emitted from a light casting unit 1 including an LED as its light source is cast into a sample cell 2, and a photodetector 3 is placed at a position where the resultant passing light can be detected. The LED is driven to blink, and a data extracting section 71 extracts data obtained in a period in which the LED is turned on, as data (absorbance data) in which absorption of light by the sample solution is reflected. Moreover, in the case where a fluorescent component is contained in the sample solution, fluorescent light is emitted by the cast light serving as excitation light. Even after the excitation light ceases, the emission of the fluorescent light continues for a short time, and hence the data extracting section 71 extracts data obtained immediately after the LED is turned off, as data (fluorescence data) in which the fluorescent light is reflected. An absorbance computing section 72 calculates absorbance based on the absorbance data, and a fluorescence computing section 73 calculates fluorescence intensity based on the fluorescence data. Accordingly, it is possible to simultaneously perform an absorbance measurement and a fluorescence measurement on one sample while using one photodetector and thus simplifying the configuration of an optical system.

Description

Technical field [0001] The invention relates to an optical analyzer for performing absorbance measurement and fluorescence measurement on a sample in parallel. Background technique [0002] In order to detect components in a liquid sample that has been separated by a liquid chromatograph (LC), absorbance measurement using an ultraviolet-visible spectrophotometer, a photodiode array (PDA) detector, or the like is usually performed. In addition, for fluorescent components and fluorescently labeled components, in some cases, fluorescence measurement using a fluorescence spectrophotometer is performed. Although the components that can be used for fluorescence measurement are limited, in general, the sensitivity of fluorescence measurement is much higher than that of absorbance measurement. Therefore, especially in fields involving a strong demand for microanalysis, such as life sciences, drug development, and environmental measurement, there is a strong demand for absorbance measure...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/64
CPCG01N21/31G01N21/64G01N2201/061G01N21/255G01N21/645G01N2021/641G01N2201/06153
Inventor 长井悠佑小川佳祐渡边真人神宫句实子藤原理悟山崎智之
Owner SHIMADZU SEISAKUSHO CO LTD
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