Preparation method of mulberry protein active peptide

Abstract

The invention discloses a preparation method of mulberry protein active peptide. The method comprises the following steps that mulberry leaves and mulberry bark are washed, dried and smashed, a treating agent prepared from magnetic graphene oxide, dilute acid and hydrogen peroxide is added for digestion, the magnetic graphene oxide is recycled through a magnet, the pH value is adjusted to be 4.5-5.5, precipitate is obtained through centrifugation, water is added into the precipitate for dissolving and filtration, filtrate contains mulberry protein solution, heating treatment is carried out, cooling is carried out, the pH value is adjusted to be 7-8.5, protease is added for heat preservation, the product is placed in an ultrasonic field for ultrasonic assisted enzymolysis, high-temperature heating is carried out for inactivating enzyme, centrifugation is carried out, supernate is collected, decoloring, suction filtration, ultrafiltration, dialysis, ion exchange and concentration are carried out, and the mulberry active peptide is obtained. Mulberry leaves and mulberry bark are adopted as raw materials for preparing the active peptide, and the raw materials are agricultural waste, are low in cost and are of great significance in environmental protection and waste resource utilization. The mulberry protein extraction content and mulberry protein active peptide conversion efficiency are higher compared with a conventional method, and physical and chemical property is stable.

Description

technical field [0001] The invention belongs to the fields of medicine and biochemical industry, and in particular relates to a preparation method of mulberry active peptide. Background technique [0002] Peptides are compounds in which two or more amino acids are connected by peptide bonds, which play important physiological roles in the human body and exert physiological functions. Active polypeptides are called active peptides, also known as biologically active peptides or biologically active polypeptides. Active peptide is a general term for more than one thousand peptides (such as soybean peptide, sea cucumber peptide, etc. are one of the active peptides). It plays a key role in human growth and development, metabolism, disease, aging and death. Active peptides are the most important active substances in the human body. It is precisely because of the increase or decrease of its secretion in the body that human beings have a cycle of infancy, childhood, adulthood, old...

AI Technical Summary

Problems solved by technology

[0006] Purpose of the invention: In order to overcome the difficulties in the extraction of mulberry protein and the low extraction content of mulberry active peptide in the prior art, the present invention provides a preparation method of mulberry active peptide to effectively solve the problems of mulberry protein extraction and mulberry active peptide. To solve the problem of difficult peptide preparation, expand the source of raw materials for active peptide production, avoid environmental pollution caused by redundant mulberry leaves and mulberry bark, mulberry leaves incinerated or discarded, and achieve greater social and economic benefits

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