Tissue culture rapid propagation method for chirita liguliformis

Abstract

The invention discloses a tissue culture rapid propagation method for chirita liguliformis. The tissue culture rapid propagation method comprises the following steps: explant preparation, primary culture, secondary culture, rooting culture, transplanting and acclimating, and the like, and specifically comprises the following steps: treating a tender leaf to obtain an explant, performing primary culture to produce an adventitious bud and obtain a young plant; cutting a leaf of the young plant into small pieces to serve as explants, and performing secondary culture to obtain robust plants; then, transferring the plants into a rooting culture medium for culturing to obtain rooted complete plants; finally, acclimating to obtain transplanting stocks. According to the tissue culture rapid propagation method for the chirita liguliformis, the breeding speed of seedlings are effectively and rapidly improved, and the propagation quality of the seedlings is ensured; a large amount of high-quality seedlings are provided for the chirita liguliformis serving as an indoor ornamental flower and a garden shade-tolerant flower; the propagation coefficient is high; meanwhile, the transplanting survival rate reaches 95 percent or higher.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of Lettuce linguica labialis. Background technique [0002] Chirita liguliformis W.T.Wang is a perennial herb of the genus Chirita liguliformis in the family Gesneriaceae. Tongue column labial column lettuce with thick rhizomes, leaves basal, opposite, 2-11 cm long, 4-9 mm wide, pubescent. Leaves thick papery, elliptic. Inflorescences about 2, 2-3 times branched, each inflorescence about 7 flowers; peduncle 20 cm long, pubescent, flowering in July; bracts narrowly triangular, about 3.5 mm long, 1.1 mm wide, short Pills; pedicels 2.5-4 cm long, pubescent. Calyx 5-lobed to base, lobes linear-lanceolate, 3-3.5 mm long, 1 mm wide, pubescent outside, glabrous inside. Corolla lavender, about 2.2 cm long, sparsely pubescent outside, pubescent inside below upper lip; tube about 1.5 cm long, mouth about 6 mm in diameter. Capsule li...

AI Technical Summary

Problems solved by technology

[0003] The anthocyanins of L. lingulae are elegant, the leaves are thick, very shade-tolerant, and easy to manage. It has a high market value to be developed as indoor ornamental flowers and landscaping shade-tolerant flowers. However, wild resources are very scarce and it is difficult to satisfy the market. It is used as a practical need for ornamental flowers and landscaping

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