A kind of double-layer embedding artificial seed production method of Zhuge Cai

Abstract

The invention discloses a making method of two-layer embedded artificial seeds of orychophragmus violaceus, and belongs to the field of biotechnology and agricultural engineering. The method comprises the steps of bud cluster inducing of the orychophragmus violaceus, bud propagation and cultivation and bud embedding and artificial seed capsule preparation; in the bud embedding and artificial seed capsule preparation process, an endopleura embedding solution is sodium alginate hydrosol which contains nano-titanium dioxide nTiO2, NAA, an MS medium and saccharose and has the mass concentration of 6%, and a testa embedding solution is a sodium alginate hydrosol which contains nano-titanium dioxide nTiO2 and nano-silicon dioxide nSiO2 and has the mass concentration of 7% or 8%. According to the made artificial seeds, a bactericide and nutrient substances can be separated through a two-layer embedding technology, and meanwhile the mechanical strength of endopleura and testa is adjusted; the artificial seeds have the advantages of being high in rooting and sprouting rate, low in contamination rate, low in toxic action on explants and the like in a bacterial environment; by means of the two-layer embedding technology, the making technology of the artificial seeds of the orychophragmus violaceus can be further pushed to practical production and application.

Description

technical field [0001] The invention belongs to the fields of biotechnology and agricultural engineering, and in particular relates to a method for making artificial seeds of zhuge kale embedded in double layers. Background technique [0002] Zhugecai, also known as Eryuelan, is a plant of the genus Zhugecai in the family Cruciferae, an annual or biennial herb, widely distributed in most parts of the country, resistant to cold, drought, and shade, and has few pests and diseases. The flowers are purple or white, and their life history is from September to June of the next year. Even in the cold winter, they can still keep green and cover the ground well. It is a rare flower in early spring and green in winter in the northern region. The ground cover plant is very popular among people. In the fatty acid composition of the seeds of Zhugecai, the content of erucic acid and linolenic acid is low, and the content of palmitic acid, oleic acid and linoleic acid is 89.8% of the tota...

AI Technical Summary

Problems solved by technology

[0004] At present, the single-layer embedded artificial seed technology has made some progress. The single-layer embedded Zhuge vegetable artificial seed added with nano-titanium dioxide can germinate in a bacterial environment, but problems such as insufficient antibacterial ability and low germination rate still restrict artificial seeds. technological development

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