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Extraction technology of total flavonoids with anti-AD (Alzheimer's disease) activity in leaves of red raspberry

An extraction process and a technology of total flavonoids, which are applied in the field of plant medicine, can solve the problems of the enrichment process and anti-senile dementia activity of total flavonoids in red raspberry leaves that have not been developed and utilized, and have achieved significant Aβ protein aggregation activity, The effect of low pollution in the production process and reasonable design

Inactive Publication Date: 2016-11-23
BAIYIN HAIRUIDA BIOCHEM TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research, development and utilization of red raspberry mainly focus on the fruit of red raspberry, which is favored by researchers because of its rich nutritional components and active substances, while raspberry leaves are mostly discarded and not developed and utilized. There are no related reports on the enrichment process and anti-senile dementia activity of total flavonoids in red raspberry leaves

Method used

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  • Extraction technology of total flavonoids with anti-AD (Alzheimer's disease) activity in leaves of red raspberry
  • Extraction technology of total flavonoids with anti-AD (Alzheimer's disease) activity in leaves of red raspberry
  • Extraction technology of total flavonoids with anti-AD (Alzheimer's disease) activity in leaves of red raspberry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The enrichment of embodiment 1 red raspberry leaf total flavonoids

[0024] Take 1000g of red raspberry leaves and pulverize them, add 8000mL 60% ethanol solution, reflux extraction for 2 hours, add 3000mL 60% ethanol solution to the filter residue after reflux extraction for 1 hour, combine the two filtrates, recover ethanol under reduced pressure, and obtain extract. Suspend this extract with 1000mL water, apply D101 macroporous resin, elute with water for 5 volumes, and then elute with ethanol solution with a volume fraction of 60% until the effluent is nearly colorless, collect the eluent, and recover under reduced pressure Ethanol, spray drying to obtain dry powder, to obtain 95.2g of total flavonoids.

[0025] The FeCl3 color reaction was positive, and the HCl-Mg reaction was positive, and the obtained extract was determined to be total flavonoids. Visible spectrophotometry (Appendix V of Chinese Pharmacopoeia One) A ), taking rutin (self-made, purity 98%) as re...

Embodiment 2

[0047] The enrichment of embodiment 2 red raspberry leaf total flavonoids

[0048] Take 95.2 g of the crude product flavonoids prepared in Example 1, add alcohol to dissolve, mix with 2 times the weight of polyamide filler, mix and dry the sample at room temperature, take 5 times the weight of polyamide filler as column filler, and elute with 15% ethanol solution After 5 volumes, elute with 80% ethanol solution until the effluent is colorless, collect the ethanol eluate, recover ethanol under reduced pressure, concentrate and dry to obtain 62.8 g of total flavonoid extract with higher purity.

[0049] Visible spectrophotometry (Appendix V of Chinese Pharmacopoeia One) A ), taking rutin (self-made, purity 98%) as reference substance, and adopting sodium nitrite-aluminum nitrate-sodium hydroxide colorimetric method, according to the reaction of flavonoids and aluminum salts to generate a red complex, the detection wavelength is 510nm, The detection result shows that the total f...

Embodiment 3

[0051] The enrichment of embodiment 3 red raspberry leaf total flavonoids

[0052] Take 62.8 g of the flavonoids prepared in Example 2, add water to suspend, apply CHP20 resin, elute with water for 5 volumes, and then elute with 55% ethanol until the effluent is colorless, collect the ethanol eluate, and depressurize The ethanol was recovered, concentrated and dried to obtain 33.8 g of high-purity refined flavonoid extract.

[0053] Visible spectrophotometry (Appendix V of Chinese Pharmacopoeia One) A ), taking rutin (self-made, purity 98%) as reference substance, and adopting sodium nitrite-aluminum nitrate-sodium hydroxide colorimetric method, according to the reaction of flavonoids and aluminum salts to generate a red complex, the detection wavelength is 510nm, The detection result shows that the total flavonoid content of the obtained fraction is 82%. Through the experiment of inhibiting Aβ protein aggregation, refer to Example 1 for the specific experimental method.

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Abstract

The invention discloses an extraction technology of total flavonoids with anti-AD (Alzheimer's disease) activity in leaves of red raspberry. The extraction technology comprises the following steps of soaking the raw material of leaves of red raspberry by 50-100% ethanol solution, heating, refluxing and extracting, relieving pressure and concentrating, so as to obtain a crude extract; mixing suspension by water, and diluting by macroporous resin and the water and ethanol system, so as to primarily obtain crude total flavonoids; using polyamide and CHP20 resin column chromatography to obtain higher-purity total flavonoids. The extraction technology has the characteristics that the raw material is a byproduct of red raspberry, namely leaves of raspberry, so that the cost is low; the extracted total flavonoids can obviously inhibit the Abeta protein gathering activity, and present good anti-AD activity in the water maze test of AD model rat; the design is reasonable, the technology is simple, the cost is low, the energy consumption is low, and the pollution in the production process is low.

Description

technical field [0001] The invention relates to the technical field of plant medicine, in particular to an extraction process of total flavonoids with anti-AD activity in red raspberry leaves. Background technique [0002] Alzheimer's disease is a non-specific disease syndrome. The main symptoms of Alzheimer's disease (AD) are the impairment of cognitive function and the ability of memory and language. . The lesions of AD have not been fully clarified. Beta-like amyloid protein (Aβ) is considered to be a key inducing factor in the occurrence and development of AD, which can cause memory function impairment and pathological changes in brain tissue. Now there are many treatments for AD. Among them, cholinesterase inhibitors are still the treatment of choice. However, these drugs can only improve symptoms such as cognitive and memory dysfunction in patients, and have a single target, and cannot fundamentally block the development of AD. Therefore, it is still important for me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P25/28
CPCA61K36/73A61K2236/333A61K2236/39A61K2236/51A61K2236/55
Inventor 刘战朋
Owner BAIYIN HAIRUIDA BIOCHEM TECH CO LTD
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