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Anti-CD3 single-domain antibody

A single-domain antibody, bispecific antibody technology, applied in recombinant DNA technology, anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, anti-animal/human immunoglobulin, etc., can solve the problem of anti-CD3 Problems such as single antibody form

Inactive Publication Date: 2016-11-23
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The anti-CD3 antibody in the prior art has a single form and cannot meet the actual needs, so it is necessary to develop more anti-CD3 antibodies to provide more options for the production and design of functional proteins

Method used

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Embodiment Construction

[0016] The present invention will now be further explained in conjunction with the following experiments and accompanying drawings. It should be noted that these experimental examples and accompanying drawings should not be construed as limiting the present invention.

[0017] 1 strategy

[0018] The present invention developed an anti-CD3 single-domain antibody through phage display technology, and carried out the following steps: animal immunity and immune response testing, construction of sdAb phage display library, phage display panning, FASEBA screening and FACS verification.

[0019] 2 materials

[0020] Antigen protein: CD3-His protein (MP-5)

[0021] Antigen cell line: Jurkat target cells, KPCN control cells

[0022] TRIzol® Reagent (Ambion, Cat. No. : 15596-026)

[0023] PrimeScript TM 1st Strand cDNA Synthesis Kit (Takara, Cat. No. : 6110A)

[0024] Sfi I enzyme (NEB, Cat. No. : R0123S)

[0025] Host bacteria: E. coli SS320

[0026] Ampicillin, 100 mg / m...

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Abstract

The invention provides an anti-CD3 single-domain antibody of which an amino acid sequence is selected from SEQ ID NO. 1 and SEQ ID NO. 3. The invention further provides a nucleotide sequence for coding the anti-CD3 single-domain antibody, an expression vector containing the nucleotide sequence and a host cell. The anti-CD3 single-domain antibody provided by the invention can resist a human CD3 epsilon N-end part and can be used for CD3 determination as well as other functional proteins needing CD3, like a bi-specific antibody.

Description

technical field [0001] The present invention relates to anti-CD3 single domain antibody (sdAb) and its amino acid and nucleotide coding sequence. The present invention also relates to expression vectors and host cells comprising the nucleotide coding sequence. Background technique [0002] Since the advent of monoclonal antibody technology in 1975, antigen-specific antibodies have transformed many aspects of biology and medicine. In addition to being important research tools, the availability of monoclonal antibodies has opened the way for the development of diagnostics and new treatments for human diseases. However, monoclonal antibodies have technical limitations and disadvantages in the range of applications, such as expensive mammalian-based expression systems. [0003] Over the past decades, a series of new technologies and approaches have been used to improve the performance of traditional antibodies, especially in tumor therapy. For example, adding toxic compounds ...

Claims

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Application Information

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IPC IPC(8): C07K16/28C12N15/13C12N15/70C12N1/21
CPCC07K16/2809C07K2317/569
Inventor 李庆王忠
Owner SUN YAT SEN UNIV
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