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Lactic acid bacteria agent suitable for grass silage and application thereof

A technology of lactic acid bacteria and bacterial agents, applied in the direction of application, microbial-based methods, bacteria, etc., to achieve the effect of extending the shelf life, increasing the content, and increasing the content of acetic acid

Active Publication Date: 2016-11-23
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Homofermentative lactic acid bacteria use water-soluble carbohydrates (WSC) to produce more lactic acid and improve the quality of silage fermentation, but the ability to inhibit aerobic deterioration is generally poor

Method used

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  • Lactic acid bacteria agent suitable for grass silage and application thereof
  • Lactic acid bacteria agent suitable for grass silage and application thereof
  • Lactic acid bacteria agent suitable for grass silage and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Isolation and Identification of Lactobacillus plantarum (Lactobacillus plantarum) ZRR and Lactobacillus buchneri (Lactobacillus buchneri) BR2

[0034] 1. Separation and purification

[0035] Lactobacillus plantarum ZRR and Lactobacillus brutenii BR2 were separated and purified from ryegrass straw, which are homofermentative lactic acid bacteria and heterofermentative lactobacillus respectively. Shake for 1 hour, then 10-fold gradient dilution, take 10 -3 、10 -4 and 10 -5 Spread sample dilution on MRS solid medium, incubate for 48 hours, then take it out, pick a single colony growing on MRS according to the size, shape and color of the colony, and carry out catalase test and Gram staining . Catalase-negative and Gram-positive were tentatively identified as lactic acid bacteria, and streaked and purified twice on MRS solid medium. Stored in MRS medium with 25% glycerol at -80°C, the strains were named ZRR and BR2 respectively.

[0036] 2. Physiological and...

Embodiment 2

[0047] Application of different bacterial strains in ryegrass silage of embodiment 2

[0048] Raw material preparation: After the ryegrass is cut, make sure it is clean and free from mildew and deterioration, and cut it to 2-3cm.

[0049] Activation of strains: Inoculate cryopreserved L.plantarumZRR and L.buchneriBR2 into MRS liquid medium respectively, culture at 37°C for 18-24h, subculture 2-3 times in this way to obtain the activated L.plantarumZRR, L.buchneriBR2 .buchneriBR2 strain; the composition of the MRS liquid medium is as follows: 10g peptone, 5g beef extract, 4g yeast extract powder, 20g glucose, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g trisodium citrate, 1mL Tween 80 , 0.2g magnesium sulfate, 0.05g manganese sulfate, add 1000mL distilled water, adjust the pH to 6.5, and sterilize at 121°C for 15min.

[0050] Preparation of bacterial powder: the above-mentioned activated strains were inoculated and fermented respectively, freeze-dried, pulverized, ...

Embodiment 3

[0063] The application of the lactic acid bacteria inoculum agent of embodiment 3 different additions in elephant grass silage

[0064] Raw material preparation, strain activation, bacterial powder preparation, elephant grass silage preparation and other implementation steps refer to Example 2. In the preparation of bacterial powder, according to the results of Example 2, the freeze-dried powder of L.plantarumZRR and L.buchneri BR2 is prepared according to 2 :1 ratio, so that the number of viable bacteria in the freeze-dried powder of lactic acid bacteria can reach 1.2×10 10 cfu / g. The prepared bacterial powder was respectively mixed with 0.01‰, 0.02‰, 0.04‰, 0.1‰, 0.2‰ (ie 1.2×10 5 , 2.4×10 5 , 4.8×10 5 , 1.2×10 6 , 2.4×10 6 cfu / g) inoculum amount inoculated in elephant grass, after adopting vacuum packing machine vacuum packing, be placed in storage room and carry out fermentation.

[0065] Detection of microbial dynamic changes in silage Elephant grass: On the 0th, 1s...

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Abstract

The invention provides a lactic acid bacteria agent suitable for grass silage. The lactic acid bacteria agent consists of lactobacillus plantarum lyophilized powder and lactobacillus buchneri lyophilized powder, wherein the lactobacillus plantarum and the lactobacillus buchneri are both separated from ryegrass straw; the lactobacillus plantarum is homofermentative lactic bacteria, and the lactobacillus buchneri is heterofermentative lactic bacteria; the two strains are collected in the China Center for Type Culture Collection on May 25th, 2016; the strain of the lactobacillus plantarum is L.plantarumZRR, and the collection number is CCTCC No:M2016281; and the strain of the lactobacillus buchneri is L.buchneriBR2, and the collection number is CCTCC No:M2016280. The lactic acid bacteria provided by the invention has the advantages of accelerating grass silage maturing, improving grass silage quality, increasing aerobic stability and the like and can be widely applied to the field of grass silage feed preparation.

Description

technical field [0001] The invention discloses a lactic acid bacteria agent suitable for pasture silage and an application thereof, belonging to the technical field of silage processing in livestock feed. Background technique [0002] Silage is a storage method that converts the fermentation substrate in raw materials into acidic substances such as lactic acid through the proliferation of lactic acid bacteria, and maintains an acidic anaerobic environment to facilitate the long-term preservation of silage crops. Silage is yellow-green in color, sour in smell, soft and juicy, and has good palatability. It is widely used in animal husbandry production, especially in ruminant breeding, and has become an important source of high-quality roughage. Compared with pasture dried into hay, pasture silage can greatly shorten the time from harvesting to storage, avoid the loss of forage caused by bad weather, and preserve the nutrients of green forage to the maximum extent. [0003] La...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K30/18C12R1/25C12R1/225
Inventor 宦海琳丁成龙刘蓓一顾洪如许能祥徐小明张霞
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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