Cell culture device applicable to comparison tests

A technology for cell culture and culture vessels, which is applied in the field of cell culture, can solve problems such as deficiencies, and achieve the effects of high accuracy, good regulation consistency, and stable structure

Active Publication Date: 2016-12-07
杭州荣泽生物科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, when conducting cell culture and comparison tests in the process, although ordinary culture devices can already provide the carrying space for the culture medium, they are not ideal in terms of reasonable space layout, overall operation convenience, and consistency of control effects. , still lacking

Method used

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  • Cell culture device applicable to comparison tests
  • Cell culture device applicable to comparison tests
  • Cell culture device applicable to comparison tests

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0036] Example 1: In the circumferential direction of the rotating tube, the drain groove, the liquid equalizing hole, the tube liquid supply hole, and the cylinder liquid supply hole are sequentially arranged in a clockwise direction. During the operation of liquid equalization control (that is, the aforementioned process of draining liquid through the liquid equalization hole to make the liquid volume in each dish consistent), the liquid drainage tank rotates clockwise with the rotating tube to align with the liquid equalization hole (during this process, the tube feeds liquid hole is not connected to the liquid supply hole of the corresponding tube), after the liquid equalization control is completed, the liquid discharge tank continues to rotate clockwise and is staggered from the liquid equalization hole. At this time, the liquid supply hole of the tube and the liquid supply hole of the corresponding cylinder are still not connected. Then, if you continue to rotate the rot...

example 2

[0037] Example 2: In the circumferential direction of the rotating tube, the uniform liquid hole, the liquid drainage groove, the liquid supply hole of the tube, and the liquid supply hole of the cylinder are arranged in a clockwise direction, and the liquid is controlled (that is, the liquid is drained through the liquid uniform hole as described above) The process of making the liquid volume in each dish consistent) During operation, the liquid discharge tank rotates counterclockwise with the rotating tube to align with the liquid equalization hole (during this process, the liquid supply hole of the tube is not connected to the liquid supply hole of the corresponding cylinder), and the liquid control volume After the completion, the liquid discharge tank rotates clockwise and is staggered with the liquid equalizing hole. At this time, the liquid supply hole of the tube and the liquid supply hole of the corresponding cylinder are still not connected. Then, if you continue to ro...

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PUM

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Abstract

The invention discloses a cell culture device applicable to comparison tests. The cell culture device comprises a pedestal and a plurality of culture vessels, wherein the culture vessels are sequentially stacked; each culture vessel comprises a vessel body, a vertical column and a liquid injection tube, and the upper and lower ends of the vertical column are respectively opened; a rotary tube penetrates through all the vertical columns, and can rotate relative to the vertical columns; the rotary tube and each vertical column form a sealing contact; and the outer side wall of the rotary tube is provided with a drainage groove. In one culture vessel, the vertical column is provided with a liquid balancing hole which communicates with the inside of the vessel body, a cell filter screen and a liquid balancing one-way valve are arranged in the liquid balancing hole, the liquid balancing one-way valve is positioned between the cell filter screen and the rotary tube, the passable direction of the liquid balancing one-way valve is from the inside to the outside of the vessel body, and the lower end of the liquid injection tube communicates with the inside of the vessel body. The cell culture device is suitable for cell culture, determination and comparison, has the advantages of reasonable spatial arrangement and stable structure, is convenient in the operational process, and has high adjusting consistency and accuracy in the test process.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a cell culture device which can be used for comparative experiments. Background technique [0002] Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. The growth of cells requires a nutrient environment, and the nutrient matrix used to maintain cell growth is called a medium. The medium can be divided into liquid medium and solid medium according to its physical state. In addition to being used in production, cell culture technology is currently used more in experiments (such as determining the parameters of group samples, and conducting comparisons and research), and liquid culture media is commonly used in experiments. In addition, cell culture requires the use of cell culture devices, such as petri dishes, incubators, etc. Cell culture in general environments can directly use petri dishes, and if so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/22
CPCC12M23/10C12M23/42C12M23/48
Inventor 陈相波雷鸣
Owner 杭州荣泽生物科技集团有限公司
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