Bdellovibrio freeze-drying preparation and preparation method thereof

The technology of freeze-dried preparation and leech vibrio, which is applied in the field of preparation of lyophilized preparation of leech vibrio, can solve the problems such as the decline of effective components, increase and change of unsafe factors, and achieve the effect of improving activity

Inactive Publication Date: 2016-12-07
贝嘉美(天津)生物技术研发股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such preparations are usually in liquid state at present, because in this type of liquid preparation, with the continuous growth and reproduction of the own bacteria, the metabolic products in the liquid preparation will continue to accumulate, the pH value will gradually change, and the miscellaneous bacteria in the product It will also proliferate day by day, and the toxic and harmful factors will accumulate more and more. Therefore, the active ingredients of the product will inevitably decline, and the unsafe factors will increase day by day.

Method used

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  • Bdellovibrio freeze-drying preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation of embodiment 1 Bdellovibrio bacterial liquid

[0027] The Bdellovibrio is exemplified as Bdellovibrio (Bdellovibrio sp.) BDS02, and the preservation number is CCTCC NO: M209170;

[0028] Described Bdellovibrio microbial preparation obtains through the following steps:

[0029] 1. Preparation of host bacterium concentrate: pick Escherichia coli single colony, inoculate it in the nutrient broth liquid medium, cultivate to obtain the culture solution, then the culture solution is centrifuged, discard the supernatant, and use 3mL of the precipitation collected for every 100mL culture solution Suspend in DNB liquid medium to obtain concentrated solution of host bacteria, store at 4°C for later use;

[0030] 2. the preparation of Bdellovibrio microbial preparation: mix according to Bdellovibrio: host concentrated solution: the volume ratio of DNB liquid culture medium 1:1:50 ratio, constant temperature culture, add once host concentrated solution every 24h, ...

Embodiment 2

[0032] Example 2 Preparation of freeze-dried refrigerant 1

[0033] Add an appropriate amount of water to the milk powder and heat it to dissolve, then add sucrose, trehalose, and sodium glutamate, so that the concentrations of each component are skim milk powder 11% w / v, glycerin 15% v / v, and sucrose 10% w / v , trehalose 17% w / v, sodium glutamate 2% w / v, galactose 5% w / v, mix well, adjust the pH value to 8.0, sterilize, keep cold for later use.

Embodiment 3

[0034] Example 3 Preparation of lyophilized refrigerant 2

[0035] Add an appropriate amount of water to the milk powder and heat it to dissolve, then add sucrose, trehalose, and sodium glutamate, so that the concentrations of each component are skim milk powder 10% w / v, glycerin 15% v / v, and sucrose 8% w / v , trehalose 14% w / v, sodium glutamate 1.5% w / v, galactose 5% w / v, mix well, adjust the pH value to 8.0, sterilize, cool and set aside.

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Abstract

The invention discloses a bdellovibrio freeze-drying preparation and a corresponding freeze-drying method. The method comprises the following steps of preparing bacteria solution according to a conventional culture method of bdellovibrio bacteriovorus to obtain a bdellovibrio bacteriovorus water preparation; mixing the bdellovibrio bacteriovorus water preparation with a freezing agent to obtain a mixed solution to be subjected to freeze drying; putting the mixed solution to be subjected to freeze drying into a freeze-drying container to be subjected to freeze-drying so as to obtain the bdellovibrio bacteriovorus freeze-drying preparation. Through data calculation, the shelf life of the bdellovibrio bacteriovorus freeze-drying preparation is much longer than that of a freeze-drying product obtained by a freeze method and a corresponding freeze agent in the prior art; the shelf life of the bdellovibrio bacteriovorus freeze-drying preparation is obviously prolonged. In addition, a protection agent provided by the invention has low price, and is suitable for large-scale popularization.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of a Bdellovibrio freeze-dried preparation. Background technique [0002] Without water, there will be no life, and there will be no human beings. However, with the increase of the social population, the demand for life is increasing, and the emissions are also increasing. Therefore, people need food for life, and food production requires pesticides and chemical fertilizers. A large number of chemical fertilizers and pesticides are used to increase production and harvest. Excessive use of chemical fertilizers and pesticides causes water pollution. Toxic and harmful substances such as arsenic, cadmium, and cyanide in pesticides flow into ditches, ponds, dams, and rivers and lakes when it rains. , pollute water resources, make green water smelly, cause fish and shrimp not to grow, spread harmful microorganisms, produce germs and viruses, pollute the ecological envi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/01
CPCC12N1/20C12N1/04
Inventor 苏永鹏
Owner 贝嘉美(天津)生物技术研发股份有限公司
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