Method for cultivating, rapidly propagating and regenerating tissues of seedlings of radix aconiti lateralis preparata

Abstract

The invention discloses a method for cultivating, rapidly propagating and regenerating tissues of seedlings of radix aconiti lateralis preparata. Stem fragments with auxiliary buds, stem fragments, sterile leaves and seeds of the radix aconiti lateralis preparata are used as explants, rapid propagation systems and seed sterile cultivation and regeneration systems for optimizing the radix aconiti lateralis preparata are built by the aid of links of disinfection conditions, bud induction, bud multiplication, rooting and transplanting, callus induction, multiplication and differentiation and the like, and technical support can be provided for industrializing seedling growing. The method includes a process for rapidly propagating the radix aconiti lateralis preparata, a process for regenerating and propagating the radix aconiti lateralis preparata and a process for rapidly growing the seedlings of the radix aconiti lateralis preparata. The process for rapidly propagating the radix aconiti lateralis preparata includes a technology for rapidly propagating the stem fragments of the radix aconiti lateralis preparata and a technology for rapidly propagating the seeds; the process for regenerating and propagating the radix aconiti lateralis preparata includes a technology for regenerating and propagating the stem fragments and adventitious roots of the radix aconiti lateralis preparata and a technology for regenerating and propagating the sterile leaves of the radix aconiti lateralis preparata. The stem fragments and the adventitious roots of the radix aconiti lateralis preparata are used as explants in the technology for regenerating and propagating the stem fragments and adventitious roots of the radix aconiti lateralis preparata. The sterile leaves of the radix aconiti lateralis preparata are used as explants in the technology for regenerating and propagating the sterile leaves of the radix aconiti lateralis preparata. The method has the advantages that the radix aconiti lateralis preparata seedling rapid propagation and growing efficiency can be obviously improved, the seedling growing periods can be obviously shortened, technical support can be provided for production application of radix aconiti lateralis preparata seedling rapid propagation and growing, and problems in the aspect of seedling propagation and growing on current markets can be effectively solved.

Description

technical field [0001] The invention belongs to the technical field of aconitum propagation, and in particular relates to a method for rapid propagation and regeneration of aconite seedling tissue culture. Background technique [0002] Aconite (Radix Aconiti Lateralis Preparata) is the root of Aconitum carmichaelii Debx., a plant of Ranunculaceae. It tastes pungent, sweet, and hot in nature; it is poisonous; The function of expelling wind-cold-damp evil. It is suitable for yang deficiency, cold limbs, weak pulse, impotence, cold uterus, vomiting and diarrhea due to deficiency and cold, edema due to yin and cold, arthralgia due to cold and dampness, cold pain in trusted subordinates, and exogenous pathogenic factors due to yang deficiency. Aconite is known as "the first medicine for recovering yang and rescuing adversity" in traditional Chinese medicine. Its medicinal value was first recorded in "Shen Nong's Materia Medica". The four-inch one is Tianxiong, the one that grow...

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Problems solved by technology

[0004] The object of the present invention is to provide a method for rapid propagation and regeneration of Aconitum aconitum seedling tissue culture, aiming to solve t

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