A fluorescent probe for detecting hydrogen peroxide and its application
A fluorescent probe, hydrogen peroxide technology, applied in the field of analytical chemistry, can solve the problem of poor selectivity of oxidizing substances with different sensitivity, and achieve the effects of good selectivity, good imaging effect and low cytotoxicity
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[0031] Synthesis of Intermediate A-1: To a suspension of 4-diethylaminoketoacid (6.0 g, 19.1 mmol) and p-chlororesorcinol 15 (2.8 g, 19.1 mmol) in TFA (20 mL), molecular sieves were added (2.0 g), then heated to 90 ºC and stirred for 6 hours. After the reaction, the mixture was cooled to room temperature and concentrated to obtain a crude product, which was recrystallized in a 1:1 mixed solution of petroleum ether: ethyl acetate to obtain a red solid A-1 (7.0 g, 95%).
[0032] Synthesis of probe ACR: To a solution of 2-(azidomethyl)benzoic acid (252.25 mg, 1.43 mmol) in dichloromethane (5 mL) was added EDCI (341.50 mg, 1.78 mmol), DMAP (72.54 mg, 0.59 mmol ) and 4-diethylaminoketoacid (500.0 mg, 1.19 mmol), then heated to room temperature and stirred for 12 hours. After the reaction was complete, the resulting solution was poured into water (30 mL) and extracted with dichloromethane (3 × 20 mL), the combined organic layers were washed with saturated brine, and then washed w...
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