Preparation of paper-based electrochemiluminescence converter and its application in the detection of carcinoembryonic antigen
A carcinoembryonic antigen and electrochemical technology, applied in the field of preparation of three-dimensional paper-based converters, can solve problems such as poor conductivity and lack of target recognition elements, and achieve the effects of simple preparation process, high accuracy of measurement results, and low cost
Inactive Publication Date: 2018-11-13
UNIV OF JINAN
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Problems solved by technology
[0005] In order to solve the problems of poor conductivity and lack of specific target recognition elements in the process of protein detection, and further expand the detection limit of tumor markers, we introduced molecular transformation technology into the detection of tumor markers, and transformed the input protein signal In order to output the signal of the aptamer chain, combined with the cheapness, portability, and easy modification of the paper chip, the detection of low-concentration tumor markers can be achieved by means of electrochemiluminescence.
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Embodiment 1
[0028] Preparation of paper-based electrochemiluminescence converter and its application in the detection of carcinoembryonic antigen:
[0029] (1) Design the hydrophobic wax batch printing pattern of the paper-based ECL converter on the computer, which are respectively defined as paper chip 1 and paper chip 2;
[0030] (2) Use a wax printer to print hydrophobic wax batch patterns on A4-sized paper chip 1 and paper chip 2, and then place the paper chips printed with hydrophobic wax batch patterns in an oven to heat until the wax melts, making it on the paper chip A hydrophobic region is formed on the
[0031] (3) Use screen printing to print the reference electrode and counter electrode printing patterns sequentially on the hydrophilic area on the left side of the front side of the paper chip 1 obtained in step (2), wherein the right side of the front side is the hydrophilic working area, The left side of the front side is the reference area, and the back side of the working ...
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The invention discloses a preparation method of a paper-based electrochemical luminescence converter and applications of the electrochemical luminescence converter in carcino-embryonic antigen detection. Through wax printing technology, screen printing technology and the functionalization of paper chips, an input signal of protein is converted into an output signal of a specific aptamer chain to further cause the change of luminol electrochemical luminescence intensity, so that the high-sensitive and portable detection of low-concentration tumor markers can be realized.
Description
technical field [0001] The invention relates to the technical field of electrochemiluminescence analysis and detection, in particular to the preparation of a three-dimensional paper-based converter suitable for electrochemiluminescence analysis. Background technique [0002] Under the action of carcinogenic factors in normal cells, the genes in the cells will change, and the growth regulation of cells will also change accordingly, which will lead to monoclonal abnormal proliferation and canceration of normal cells. In recent years, the incidence and mortality of cancer have been increasing day by day, and people are full of fear and worry about it. Therefore, early warning of cancer has become a major research goal for scientific researchers. [0003] Tumor markers are a class of substances that reflect the presence or absence of tumors. Their existence and quantitative changes can provide the properties of tumors. Therefore, the detection of tumor markers plays an important...
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IPC IPC(8): G01N21/76G01N33/574
CPCG01N21/76G01N33/57473
Inventor 李丽张彦黄煜真梁琳琳葛慎光颜梅于京华
Owner UNIV OF JINAN