Single-step seedling culture method for pleione bulbocodioides through tissue culture

A tissue culture and culture method technology, applied in the field of agricultural science and technology, can solve problems such as low reproduction coefficient and difficult germination, and achieve the effects of reducing production costs, solving yellowing, and shortening tissue culture time.

Active Publication Date: 2017-01-18
GUIZHOU CROP VARIETIES RESOURCE INST
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Problems solved by technology

Nowadays, the cultivation technology of solitary orchid is mature, but the problem of seedlings has not been solved. The seeds of solitary orchid are very small, and it is difficult to germinate under natural

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  • Single-step seedling culture method for pleione bulbocodioides through tissue culture

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Embodiment 1

[0015] Embodiment 1 of the present invention: the one-step seedling cultivation method of Suanlanlan tissue culture,

[0016] Test site: Laboratory of Modern Chinese Medicinal Materials Research Institute, Guizhou Academy of Agricultural Sciences

[0017] 1) Acquisition of high-quality seeds: select the pseudobulbs with full buds, large size, and no disease, plant them from November to February of the next year, and artificially pollinate them after flowering from March to April;

[0018] 2) Harvesting and selection of capsules: 190-210 days after pollination, select plump yellow capsules and harvest them in good time;

[0019] 3) Aseptic sowing: After the capsules are harvested, rinse the surface of the fruit with water first, then wipe the surface of the capsules with alcohol for disinfection treatment, and sow the seeds evenly on the surface of the sowing medium under aseptic conditions, and place the seeding bottle in the cultivation room Seeds begin to germinate into gre...

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Abstract

The invention discloses a single-step seedling culture method for pleione bulbocodioides through tissue culture. The advantages of large pleione bulbocodioides capsule seed quantity is fully utilized, seeds are used as explants to establish a tissue culture and rapid propagation technology suitable for single-step pleione bulbocodioides seedling. The tissue culture time is greatly shortened through an MSN culture medium, the problems of yellowing, difficult rooting and excessive stem and leaf growth of tissue cultured seedlings can be effectively solved, the production cost is greatly reduced, the survival rate of propagation is improved, and the method is a technology worthy of popularization.

Description

technical field [0001] The invention relates to the field of agricultural science and technology, in particular to a one-step seedling cultivation method for tissue culture of orchid albicans. Background technique [0002] Pleione bulbocodioides (Franch.) Rolfe, also known as Pleione bulbocodioides (Franch.) Rolfe, is a perennial plant of the genus Pleione bulbocodioides (Franch.) Rolfe. , The function of reducing swelling and dissipating stagnation. In recent years, it has received increasing attention due to its anticancer effects. With the development of the medicinal value of Sulilan, the price continues to rise, and the wild resources are on the verge of extinction. Nowadays, the cultivation technology of solitary orchid is mature, but the problem of seedlings has not been solved. The seeds of solitary orchid are very small, and it is difficult to germinate under natural conditions. The main method of production is to use the division method to reproduce. The reproduc...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H1/02
CPCA01H1/02A01H4/001A01H4/008
Inventor 张丽娜朱国胜桂阳王沁陈娅娅罗安阔毛伟胜
Owner GUIZHOU CROP VARIETIES RESOURCE INST
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