A kind of method of avocado tissue culture and rapid propagation

Abstract

The present invention proposes a method for tissue culture and rapid propagation of avocado, comprising the following steps: (1) disinfection of seeds, no cracking of seeds: disinfection treatment with volume concentration 70-80% ethanol solution, or seed cracking: adoption of volume concentration 70% ~80% ethanol solution and 0.2~0.8% sodium hypochlorite solution disinfection treatment with a mass concentration containing 1-2 drops of Tween; (2) cutting the seeds, taking out the embryos, removing other tissues outside the embryos to obtain explants; (3 ) inoculate the explants in the induction medium, and then carry out dark and light culture successively to obtain adventitious buds; (4) cut the adventitious buds into bud pieces, and after multiplication and culture, include gradient culture and rooting by using transition medium The medium is cultured for rooting to obtain tissue cultured seedlings; (5) hardened seedlings are transplanted. The invention realizes the rapid tissue culture and breeding method of the isolated avocado, not only increases the reproduction coefficient of the avocado seedlings, but also solves the problems of difficulty in rooting of the avocado, and the tissue cultured seedlings are strong and have a high survival rate.

Description

A kind of method of avocado tissue culture and rapid propagation technical field The present invention relates to the technical field of plant propagation, particularly a kind of method of oil pear tissue culture and rapid propagation. Background technique Avocado (Persea americana Mill), also known as avocado, avocado, originates in Central America and Mexico wet It is a famous tropical and subtropical fruit and one of the woody oil tree species. Hainan, Guizhou, Guangdong, Guangxi, Yunnan and other provinces (regions) are suitable for cultivation. Avocado is a fruit with extremely high nutritional value, containing a variety of vitamins, fatty acids, protein and Mineral elements, of which 80% of the fatty acids are unsaturated fatty acids such as linoleic acid and linolenic acid, which are essential for the human body. The absorption rate of the human body As high as 93.7%, it is known as "forest butter". Avocado is also a high-energy food and has been called "f...

AI Technical Summary

Problems solved by technology

[0003] The main factor restricting the development of avocado industrialization is the rapid breeding of healthy seedlings. Now the planting areas in various production areas suitable for avocado planting are rapidly expanding the planting area, but the lack of seedlings seriously restricts the planting area.

At present, the country mainly relies on seeds as rootstocks for propagation, and the singleness and limitations of the propagation materials make it impossible to produce healthy avocado seedlings quickly and on a large scale.

Avocado is a woody fruit tree, which restricts the rapid propagation of tissue culture seedlings, mainly due to the low rate of polybud induction, weak growth of adventitious buds, and poor rooting effect of tissue culture seedlings, resulting in long seedling breeding cycle and low survival rate

In the previous research and previous technologies, the main problem is that it cannot grow or the rooting efficiency is not high. Therefore, the rapid breeding of avocado seedlings in vitro is a bottleneck problem that needs to be solved urgently.

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