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Isolated rooting culture method for fir clone

A technology of rooting culture and rooting medium, which is applied in the field of asexual propagation of Chinese fir, can solve problems such as the difficulty of rooting tissue culture seedlings, and achieve the effects of solving the problem of difficult rooting, good social benefits, and good economic prospects

Active Publication Date: 2012-10-10
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Purpose of the invention: for the deficiencies in the prior art, the purpose of the invention is to provide a method for in vitro rooting culture of Chinese fir clones, and then solve the difficult problem of rooting of Chinese fir different fine clones under tissue culture conditions, and realize Establish a stable and efficient tissue culture system for Chinese fir to provide technical support for accelerating the industrialization of Chinese fir tissue culture seedlings

Method used

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  • Isolated rooting culture method for fir clone
  • Isolated rooting culture method for fir clone
  • Isolated rooting culture method for fir clone

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1 Bud differentiation and elongation

[0030] Each tissue culture material (Yang 062, Yang 020, Yang 061, Yang 023 and Yang 021) was cultured in the subculture medium to induce differentiation of adventitious buds. The formula of the subculture medium was 3 / 4MS+6BA0.3 mg · L -1 +NAA0.02mg·L -1 + 3% sucrose. The induced differentiated adventitious buds were transferred to MS medium without any growth regulators for elongation culture.

Embodiment 2

[0031] Example 2 induced rooting

[0032] When each adventitious bud of embodiment 1 grew to 2~3cm, it was transferred to rooting medium to cultivate its rooting. Add plant growth regulators and 2% sucrose to the rooting medium and observe the statistics. Data statistical indicators include: induction frequency of adventitious roots (%) = number of adventitious buds that grow adventitious roots / number of inoculated adventitious buds × 100%; average adventitious root number = total number of adventitious roots / number of adventitious buds that induce adventitious roots; formation ability of adventitious roots ( RFC) = average number of adventitious roots × induction frequency of adventitious roots.

[0033] In Yang 062 culture, the selection of rooting medium and plant growth regulator and the corresponding experimental results are shown in Table 1. It can be seen from the table that the medium with added NAA is the best for rooting. When the concentration of NAA-1 When the r...

Embodiment 3

[0053] Inoculate Yang061 in 1 / 4MS+NAA0.1mg·L -1 +IBA0.2mg·L -1 In the culture medium, different concentrations of activated carbon (Table 7) were added to the culture medium, and the culture method was the same as in Example 2, and the statistical results were observed after 35 days. After being inserted into the activated carbon medium, the growth of the aseptic seedlings was weak, and adventitious roots were found to grow on the 26th day. It can be seen from Table 7 that the rooting rate on the medium added with 0.02% activated carbon was 46.7, and the rooting rate continued to decrease with the increase of activated carbon concentration. %. It can be seen that activated carbon is not conducive to the rooting of fir adventitious buds.

[0054] Table 7 The effect of active carbon on the rooting of Yang 061

[0055] Activated carbon (mg / L) Number of inoculated explants number of rooted explants Rooting rate (%) 0.2 15 7 46.7 0.5 15 6 40 1....

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Abstract

The invention discloses an isolated rooting culture method for a fir clone. The method comprises the following steps of: selecting basal sprouts of a trunk of an original plant of the excellent fir clone as a tissue culture material, inoculating the basal sprouts to a successive multiplication culture medium to perform successive multiplication culture, inducing and differentiating adventitious buds, and transferring the adventitious buds to an MS (Murashige and Skoog) culture medium to perform elongation culture; when the adventitious buds are grown to 2 to 3 centimeters, transferring the adventitious buds to an induced rooting culture medium, and performing culture to obtain rooted test tube plantlets; and transplanting the rooted test tube plantlets, and mixing loess and peat soil in aratio of 3:1 to form s transplanting matrix. Compared with a fir tissue culture method in the prior art, the isolated rooting culture method for the fir clone has the advantages that: the rooting difficulty problem of tissue culture plantlets of different excellent clones of fir under the tissue culture condition is effectively solved, a stable and efficient tissue culture system of the fir is established, the highest rooting rate can reach 100 percent (onion 020), and a technical support is provided for quickening the industrialized development of the fir tissue culture plantlets.

Description

technical field [0001] The invention relates to Chinese fir asexual propagation technology, in particular to a method for in vitro rooting culture of Chinese fir clones. Background technique [0002] Chinese fir (Cunninghamia lanceolata) is an evergreen tree belonging to the gymnosperm family Taxodiaceae. It likes a warm and humid climate and grows rapidly. Chinese fir is light, soft, delicate, straight-grained, and easy to process; it can be used for construction, bridges, shipbuilding, electric welding, pit wood, wood toon, etc., and can be used as raw materials for papermaking, textiles, etc.; the bark, roots, and leaves are used as medicine to dispel wind. Dampness, astringent and hemostasis; seeds contain about 20% oil, used for making soap. [0003] Chinese fir is the main afforestation tree species and the most important commercial timber species in southern my country. It has a long history of cultivation, and its afforestation area and forest stand volume rank firs...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈金慧施季森郑仁华程强
Owner NANJING FORESTRY UNIV
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