Bispyrene-based pH (Potential of Hydrogen)-response self-assembly polypeptide nano material, and preparation method and application thereof

A nanomaterial and self-assembly technology, applied in the field of nanomaterials, can solve problems such as limiting biological applications, achieve wide application prospects, improve utilization rate, and achieve good stability

Active Publication Date: 2017-01-25
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, small molecule imaging agents and therapeutic drugs have certain hydrophobicity, which limits their biological applications
The current method for the use of small molecule imaging agents and therapeutic drugs is to encapsulate them with polymer nanomaterials, which is still challenging for the controlled release of small molecule imaging agents and drugs

Method used

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  • Bispyrene-based pH (Potential of Hydrogen)-response self-assembly polypeptide nano material, and preparation method and application thereof
  • Bispyrene-based pH (Potential of Hydrogen)-response self-assembly polypeptide nano material, and preparation method and application thereof
  • Bispyrene-based pH (Potential of Hydrogen)-response self-assembly polypeptide nano material, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] In this example, the structure of the bipyrene-based polypeptide nanomaterial is as follows:

[0046]

[0047] That is, in formula II provided by the present invention, R 1 The donor is KLVFF, R 2 The donor is a hexapeptide composed of 6 histidines, R 3 The donor is carboxy-terminated polyethylene glycol with a molecular weight of 1000 g mol -1 .

[0048] The bispyrene-based polypeptide nanomaterials are synthesized by the solid-phase synthesis method of polypeptides, using Wang's resin, according to the sequence of polypeptides, through coupling agents (nitromethylmorpholine: DMF = 5:95, volume ratio) for coupling The PEG and bispyrene compounds with carboxyl groups are also reacted according to the coupling method of amino acids, and finally obtained by cleavage with trifluoroacetic acid, rotary evaporation, and ether purification.

[0049] Mass spectrometry was performed on the bipyrene-based polypeptide nanomaterial synthesized in this example, and the result...

Embodiment 2

[0051] In this example, the bipyrene-based polypeptide nanomaterial prepared in Example 1 was self-assembled, and the pH responsiveness of the material was investigated.

[0052] First, to test the self-assembly ability of the bipyrene-based polypeptide nanomaterials, dissolve the bipyrene-based polypeptide nanomaterials prepared in Example 1 in DMSO, test its fluorescence intensity, and then add water for group assembly. After watering, mix the water and the organic phase thoroughly, and then let it stand for one minute to test its fluorescence intensity. The test results are as follows: figure 2 shown.

[0053] From figure 2 It can be seen that, when adding H to the DMSO solution of bispyrene-based polypeptide nanomaterials 2 The fluorescence increases at O, indicating that the material changes from monodisperse to aggregated.

[0054] The pH-responsive self-assembly method of the bipyrene-based polypeptide nanomaterial is as follows:

[0055] Weigh 5 mg of bispyrene-b...

Embodiment 3

[0058] The bipyrene-based polypeptide nanomaterial prepared in Example 1 was subjected to cell confocal testing to investigate its recruitment to drugs or imaging agents, the method is as follows:

[0059] Prepare cell suspension, 1 mL per confocal dish, and culture overnight. Take out the culture medium, add 1 mL of culture medium with a concentration of 20 μM polypeptide nanomaterials based on bispyrene, overnight; replace the drug with 20 μM Nile Red (Nile Red), incubate for 2 hours, wash with PBS 3 times, and perform confocal imaging experiments. Adopt 405nm laser channel, collect green light band 450-550nm, red light band 550-700nm.

[0060] The result is as Figure 4 As shown, cell spheres were co-incubated with materials and Nile Red, and single-photon confocal experiments showed that when excited at 405nm, when the range of 450-550nm was collected, the green light of bipyrene was displayed; when the range of 550-700nm was collected, it was Ni Luohong's red light, tha...

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Abstract

The invention provides a bispyrene-based pH (Potential of Hydrogen)-response self-assembly polypeptide nano material, and a preparation method and application thereof. The bispyrene-based pH-response self-assembly polypeptide nano material has a structure as shown in formulas I to IV. The bispyrene-based pH-response self-assembly polypeptide nano material has pH response, can be self-assembled into nano fiber under the environment that the pH is greater than or equal to 1.0 and smaller than 8.0 and can be assembled into nanospheres under the environment that the pH is greater than or equal to 8.0, and a hydrophobic micromolecular imaging agent and medicine can be recruited, so that the utilization rate of the micromolecular imaging agent and treatment medicine can be greatly increased; the bispyrene-based pH-response self-assembly polypeptide nano material can be converted into fiber in positions of ill tissues or cells in situ, is capable of greatly prolonging the retention time, can be used for biological imaging and treating, is capable of providing a reliable diagnosis and targeted therapy method for disease treatment, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials and relates to a polypeptide nanomaterial and its preparation method and application, in particular to a bipyrene-based pH-responsive self-assembled polypeptide nanomaterial and its preparation method and application. Background technique [0002] In recent years, the application of nanomaterials in the diagnosis and treatment of clinical diseases has received great attention. Peptide self-assembled nanomaterials are widely used in bioimaging and therapy due to their good biocompatibility and chemical diversity. Nanomaterials with different sizes and shapes have great impact on biological imaging and therapy. For example, nanoparticles, related studies have shown that when the size of nanoparticles is 10-100nm, the circulation time of nanoparticles in the body will be increased; and when the size of nanoparticles is 15-350nm, nanoparticles will be targeted and aggregated to myocardial infar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/117C07K7/06A61K9/70A61K47/42A61K9/51A61K49/00B82Y30/00B82Y40/00
CPCA61K9/5169A61K9/70A61K47/42A61K49/0021A61K49/0056B82Y30/00B82Y40/00C07K5/1024C07K7/06
Inventor 王浩罗强杨培培王磊齐国斌
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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