Temperature-sensitive injectable postoperative anti-adhesion material and preparation method thereof

An anti-adhesion and temperature-sensitive technology, which is applied in the fields of polymer materials and biomedical materials, can solve problems such as poor adhesion effect, hidden dangers of biological safety, and poor operation convenience, and achieve good biodegradability, process operation, and good integrity. sexual effect

Active Publication Date: 2017-02-01
QINGDAO CHENLAND MARINE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a temperature-sensitive injectable postoperative anti-adhesion material and its preparation method to solve the problems of poor operation convenience, poor postoperative adhesion prevention effect and in vivo use of existing anti-adhesion materials. Biological safety hazards and other issues

Method used

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  • Temperature-sensitive injectable postoperative anti-adhesion material and preparation method thereof
  • Temperature-sensitive injectable postoperative anti-adhesion material and preparation method thereof
  • Temperature-sensitive injectable postoperative anti-adhesion material and preparation method thereof

Examples

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Effect test

Embodiment 1

[0031] (1) Dissolve tamarind gum in deionized water at 25°C and stir for 12 hours to obtain a solution with a mass fraction of 1%;

[0032] (2) Centrifuge the solution obtained in step (1) at a speed of 9000 rpm for 15 minutes, and then drop the supernatant obtained from the centrifugation into anhydrous ethanol whose volume is 3 times its volume to obtain the precipitation product xyloglucan sugar;

[0033] (3) freeze-drying the xyloglucan obtained in step (2) to obtain dried xyloglucan;

[0034] (4) Dissolve the xyloglucan obtained in step (3) in a sodium acetate buffer of pH 4.5, and then add β-galactosidase derived from Aspergillus oryzae to obtain a xyloglucan concentration of 2% ( g / mL), a solution with a β-galactosidase concentration of 0.1% (g / mL);

[0035] (5) The solution system obtained in step (4) was mechanically stirred and reacted at 50°C and 500rpm for 24 hours, and then heated to 100°C for 30 minutes to terminate the enzymatic hydrolysis reaction, and then the reacti...

Embodiment 2

[0039] (1) Dissolve tamarind gum in deionized water at 15°C and stir for 48 hours to obtain a solution with a mass fraction of 2%;

[0040] (2) Centrifuge the solution obtained in step (1) at 20000 rpm for 5 minutes, and then drop the supernatant obtained from the centrifugation into absolute ethanol with a volume 10 times its volume to obtain the precipitated xyloglucan sugar;

[0041] (3) Dry the xyloglucan obtained in step (2) at a constant temperature and vacuum at a temperature of 25°C to obtain a dried xyloglucan;

[0042] (4) Dissolve the xyloglucan obtained in step (3) in sodium acetate buffer of pH 3.5, and then add β-galactosidase derived from Aspergillus oryzae to obtain a xyloglucan concentration of 3% ( g / mL), a solution with a β-galactosidase concentration of 0.15% (g / mL);

[0043] (5) The solution system obtained in step (4) was mechanically stirred and reacted at 50°C and 100rpm for 48 hours, then heated to 120°C for 15 minutes to terminate the enzymatic hydrolysis re...

Embodiment 3

[0047] (1) Dissolve tamarind gum in deionized water at 70°C and stir for 4 hours to obtain a solution with a mass fraction of 1.5%;

[0048] (2) Centrifuge the solution obtained in step (1) at 1000 rpm for 30 minutes, and then drop the supernatant obtained from the centrifugation into anhydrous ethanol twice its volume to obtain the precipitation product xyloglucan sugar;

[0049] (3) Drying the xyloglucan obtained in step (2) at a constant temperature and vacuum at a temperature of 80° C. to obtain dried xyloglucan;

[0050] (4) Dissolve the xyloglucan obtained in step (3) in a sodium acetate buffer of pH 5.5, and add β-galactosidase derived from Aspergillus niger to obtain a xyloglucan concentration of 1% ( g / mL), a solution with a β-galactosidase concentration of 0.05% (g / mL);

[0051] (5) The solution system obtained in step (4) was mechanically stirred and reacted at 50°C and 1000rpm for 12 hours, then heated to 150°C for 10 minutes to terminate the enzymatic hydrolysis reaction...

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Abstract

The invention relates to a temperature-sensitive injectable postoperative anti-adhesion material and a preparation method thereof. Gel prepared by dissolving modified xyloglucan into water is adopted as the anti-adhesion material. The temperature-sensitive injectable postoperative anti-adhesion material has the reversible temperature-sensitive behaviors of being sol at low temperature and being gel at high temperature. The invention further provides the preparation method of the injectable temperature-sensitive physical gel with the low critical gel concentration and the high gel strength. According to the preparation method, the natural polysaccharide-xyloglucan is taken as the raw material, a green enzymolysis reaction of a water system is adopted, and usage of organic reagents and generation of chemical reactions are completely avoided; the preparation method is simple, the process is easy to operate, the prepared postoperative anti-adhesion material achieves the good biocompatibility, biodegradability and in-situ injectability, the convenience of postoperative operation is improved when the material is used for preventing postoperative adhesion, and the material is an ideal postoperative anti-adhesion material. The preparation method is easy, the process is easy to operate, the raw material xyloglucan is high in reserve volume and wide in source, and commercialization is easy to achieve.

Description

Technical field [0001] The invention relates to a temperature-sensitive injectable post-operative anti-adhesion material and a preparation method thereof, and belongs to the field of polymer materials and biomedical materials. Background technique [0002] Postoperative adhesions are abnormal fibrous bands formed by connecting connective tissues and adjacent tissues or organs after damage to normal tissues in a separated state. Postoperative adhesion is a common problem in the surgical field, and it is also one of the medical problems that have not been solved in the clinic for a long time. The incidence of abdominal adhesions after general abdominal surgery is 76%, and the incidence of adhesions after open gynecological pelvic surgery is as high as 97%. Adhesion after surgery may cause serious complications and make the second operation more difficult. For example, adhesion after abdominal surgery may cause chronic pain, intestinal obstruction and other complications; adhesions...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/04A61L31/14
CPCA61L31/042A61L31/145A61L31/148A61L2400/06C08L5/14
Inventor 姚芳莲张二帅孙红
Owner QINGDAO CHENLAND MARINE BIOTECH CO LTD
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