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A method of exogenously adding gentiobiose to alleviate autotoxic damage of Panax notoginseng

The technology of gentiobiose and Panax notoginseng is applied in the field of exogenous addition of gentiobiose to relieve the autotoxicity damage of Panax notoginseng, and can solve the problems of disturbing root redox balance, continuous cropping obstacles restricting Panax notoginseng production, and reducing the content of antioxidant substances. , to overcome the decline in oxygen free radical scavenging ability, enhance antioxidant capacity, and enhance resistance

Active Publication Date: 2019-09-10
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Panax notoginseng is one of the most valuable Chinese herbal medicines in my country, but continuous cropping obstacles seriously restrict the production of panax notoginseng
Saponin autotoxic substances secreted by Panax notoginseng (such as crude saponin or saponin monomer Rg 1 ) after treating the root system of Panax notoginseng, the content of antioxidant substances in the cells is reduced, which interferes with the redox balance of the root system, resulting in the accumulation of a large amount of active oxygen in the root cells, causing oxidative damage, and eventually leading to the death of root cells
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Method used

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  • A method of exogenously adding gentiobiose to alleviate autotoxic damage of Panax notoginseng
  • A method of exogenously adding gentiobiose to alleviate autotoxic damage of Panax notoginseng
  • A method of exogenously adding gentiobiose to alleviate autotoxic damage of Panax notoginseng

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: Exogenous addition of gentiobiose enhances the antioxidant capacity of root cells

[0014] After the annual Panax notoginseng seedlings grow in the experimental shade for 7 months, select healthy Panax notoginseng plants and bring them back to the laboratory, rinse the soil on the roots with tap water, rinse them with sterile water three times, and put them into a 200mL tissue culture bottle Perform liquid culture. In order to exclude the influence of 1‰ volume percentage of methanol on the root system of Panax notoginseng, all treatments were added with 1 volume percentage of methanol. A total of 5 treatments were set up in the test, that is, the concentration of 1.0mg / L Rg 1 Treat 0h, 3h, 12h, 24h and 48h respectively. Four repetitions were set up for each treatment, and eight Panax notoginseng seedlings were placed in each repetition. The test adopts the reverse order method for treatment. All treatments are sampled after hydroponics in sterile water...

Embodiment 2

[0021] Embodiment 2: Utilize gentiobiose soaking seed treatment to improve the emergence rate of Panax notoginseng

[0022] After the Panax notoginseng seeds are collected, remove the pulp and dry the moisture on the seed surface, and prepare gentiobiose solutions with concentrations of 0, 0.1, 1.0, 10.0, 50.0, and 100.0 mg / L with deionized water with a volume percentage of 0.01% Tween 80 Soak the seeds for 10 minutes, take them out and dry the moisture on the surface of the seeds. Then the processed Panax notoginseng seeds are sown in the addition concentration that is 1.0mg / L autotoxic saponin Rg 1 In the soil, the Panax notoginseng seeds soaked with gentiobiose were planted in the new soil without autotoxic saponin as the control treatment. Three replicates were set up for each treatment, and 1000 seeds were sown in each replicate. After sowing, manage according to conventional methods, and investigate the emergence rate of the control treatment when the emergence rate re...

Embodiment 3

[0027] Embodiment 3: Utilize gentiobiose seed coating treatment to effectively relieve saponin autotoxic injury

[0028] After the Panax notoginseng seeds are collected, remove the pulp and dry the moisture on the seed surface, and use the seed coating agent Shilashi with the gentiobiose concentration of 0, 5.0, 50.0, 500.0, 2500.0, 5000.0 mg / L respectively according to the mass ratio of the drug species 1 :50 ratio mixed for coating treatment, the seed coating agent was sown after drying. Then the processed Panax notoginseng seeds are sown in the addition concentration that is 1.0mg / L autotoxic saponin Rg 1In the soil, the Panax notoginseng seeds treated only with the seed coating agent Shilax were planted in the new soil without adding autotoxic saponin as the control treatment. Three replicates were set for each treatment. 1000 seeds were sown per replicate. After sowing, manage according to conventional methods, and investigate the emergence rate of the control treatmen...

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Abstract

The invention relates to a method for relieving pseudo-ginseng autotoxicity injuries by adding exogenous gentiobiose, belonging to the technical field of plant protection. The technical scheme is as follows: carrying out seed soaking treatment on pseudo-ginseng seeds by using a 1.0-50.0 mg / L gentiobiose solution; or dissolving gentiobiose in seed coating Celest to prepare a 5.0-500.0 mg / L gentiobiose seed coating, and carrying out coating treatment on the pseudo-ginseng seeds according to the agent:seed mass ratio of 1:50; or carrying out root soaking treatment on seeds and seedlings by using a 1.0-50.0 mg / L gentiobiose solution. The exogenous antioxidant is added to enhance the resistance of pseudo-ginseng, lower the cell injuries caused by autotoxic substances in the pseudo-ginseng growth process so as to increase the root rot damage probability, and reduce the consumption of pesticides; and thus, the method is an ecological safe effective measure for relieving successive cropping obstacles.

Description

technical field [0001] The invention relates to a method for alleviating autotoxic damage of Panax notoginseng by adding gentiobiose from an exogenous source, and belongs to the technical field of plant protection. Background technique [0002] Panax notoginseng is one of the bulk valuable Chinese herbal medicines in my country, but continuous cropping obstacles seriously restrict the production of panax notoginseng. Existing studies have shown that the soil that has been planted with Panax notoginseng cannot be continuously cropped after 10 to 20 years, and the obstacle of continuous cropping has become the main factor restricting the healthy development of the Panax notoginseng industry. Over the years, many scholars have carried out in-depth research on the causes of continuous cropping obstacles of Panax notoginseng, and believe that continuous cropping obstacles are mainly related to the infection of root rot fungi, the accumulation of autotoxic substances, the deterior...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00A01C1/06A01G13/00A01G7/00A01N43/16A01P21/00
Inventor 朱书生杨敏钏有聪许彦国刘屹湘黄惠川何霞红朱有勇
Owner YUNNAN AGRICULTURAL UNIVERSITY
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