Simple high-efficiency haematococcus pluvialis nutritive cell culturing and harvesting method

Abstract

The invention belongs to the technical field of biotechnology, and especially provides a simple high-efficiency haematococcus pluvialis nutritive cell culturing and harvesting method. According to the simple high-efficiency haematococcus pluvialis nutritive cell culturing and harvesting method, a hydrocarbonate and carbonate buffer solution is added into a haematococcus pluvialis nutrient medium so as to improve carbon dioxide solubility and carbon source supply in the culture solution, and increase alga cell density; before finishing of culturing, supply of carbon dioxide is stopped, a buffer system is destroyed based on the characteristic that adsorption of hydrocarbonate is realized via alga cell photosynthesis, natural increasing of the pH value of the culture solution is realized, and falling of flagellums of swarm cells is promoted, the nutritive medium is removed quickly based on the characteristic that rapid sedimentation of haematococcus pluvialis cells at a high alkaline environment is realized, nutrient cell high efficiency harvesting is realized, and nutritive cell inducing activity is increased. The simple high-efficiency haematococcus pluvialis nutritive cell culturing and harvesting method is simple in operation, high in efficiency, and low in energy consumption, and is capable of realizing high efficiency nutritive cell harvesting, and increasing astaxanthin induction efficiency of a second stage.

Description

technical field [0001] The invention relates to the field of biotechnology, and in particular provides a simple and efficient method for culturing and harvesting Haematococcus pluvialis vegetative cells. Background technique [0002] Astaxanthin, namely 3,3′-dihydroxy-4,4′-diketone-β,β′-carotene, the chemical formula is C 40 h 52 o 4 , is a carotenoid widely found in nature. Astaxanthin from natural sources is the strongest antioxidant found in nature so far. Its antioxidant capacity is 10 times that of β-carotene and 800 times that of coenzyme Q10. It can effectively scavenge free radicals and has anti-oxidation, tumor inhibition, Enhance immune function, resist ultraviolet damage and other physiological effects. [0003] At present, in Haematococcus pluvialis, which is the main biological source of natural astaxanthin, the content of astaxanthin can reach 3.0%, or even higher, and it is regarded as a concentrated product of natural astaxanthin. A large number of studi...

AI Technical Summary

Problems solved by technology

In the first vegetative growth stage, due to the low solubility of carbon dioxide in water, the utilization of algal cell carbon source is limited, the cell density is low and there are many swimming cells with flagella, so it is difficult to collect

The existing microalgae harvesting methods, such as natural sedimentation, centrifugation, filtration, etc., have disadvantages such as low efficiency, high energy consumption, and poor applicability; while newly researched and developed flocculant harvesting methods, such as patents 201310484780.7, 201410453206.X Although the method of adding flocculant used by et al. can achieve settlement and harvest, the flocculant and algae cannot be separated, and the second step of induction culture cannot be carried out, and the above method will damage the cell activity of Haematococcus pluvialis to a certain extent. , resulting in impaired biomass and astaxanthin transformation capacity during the induction phase