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Vaginal secretion detection kit and preparation method thereof

A technology for detecting kits and vaginal secretions, which is applied in the field of vaginal secretions detection kits and its preparation, can solve the problems of simultaneous detection and other problems, and achieve the effect of simple and convenient use conditions, high efficiency and accurate detection

Pending Publication Date: 2017-02-15
GUANGZHOU HONGQI OPTICAL INSTR TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, there is no kit capable of simultaneously detecting the above four enzymes

Method used

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  • Vaginal secretion detection kit and preparation method thereof
  • Vaginal secretion detection kit and preparation method thereof
  • Vaginal secretion detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0069] The preparation method of the above-mentioned detection kit comprises the following steps:

[0070] 1) Preparation of β-glucuronidase reaction pad: dissolving polyvinylpyrrolidone and 5-bromo-4-chloro-3-indole-β-D-glucuronide in phosphate with a pH of 6.8 to 7.5 buffer solution to obtain soaking solution A; fully dissolve newborn bovine serum in a phosphate buffer solution with a pH of 6.8-7.5 to obtain soaking solution B; place the carrier in soaking solution A for 10-20 seconds, and then take it out Dry at a temperature of 40°C for 30 to 40 minutes, then fully soak in soaking solution B for 10 to 20 seconds, then take it out and dry at a temperature of 40°C for 30 to 40 minutes to obtain β-glucose Aldolidase Reaction Pad;

[0071] Described phosphate buffer saline (PBS) is NaH 2 PO 4 and Na 2 HPO 4 aqueous solution;

[0072] 2) Preparation of acetylglucosaminidase reaction pad: fully dissolve p-nitrobenzene-N-acetylglucosaminidase in anhydrous methanol to obtain...

Embodiment 1～3

[0080] Embodiments 1 to 3 disclose a reaction well for detecting β-glucuronidase, a reaction well for detecting acetylglucosaminidase, a reaction well for detecting coagulase and a reaction well for detecting proline aminopeptide The detection reagent composition in the reaction hole of enzyme, specifically as shown in table 1, prepare solution according to the parameter shown in table 2, prepare the first chromogenic agent and the second chromogenic agent according to the parameter in table 3; And through concrete implementation The method in the method prepares the detection kit:

[0081] Raw material composition and content of table 1 embodiment 1~3

[0082]

[0083]

[0084] The preparation parameter of table 2 solution

[0085]

[0086] The preparation parameters of the first chromogenic agent and the second chromogenic agent of table 3

[0087]

[0088]

[0089] Detect the detection kits obtained in Examples 1 to 3: respectively prepare β-glucuronidase s...

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Abstract

The invention discloses a vaginal fluid detection kit, which comprises a reaction device and a color developing agent, wherein the reaction device comprises a reaction hole for detecting beta-glucuronidase, a reaction hole for detecting acetylglucosaminidase, a reaction hole for detecting coagulase and a reaction hole for detecting proline aminopeptidase; when the kit is used for detecting vaginal secretion, the used diluent is normal saline. The vaginal secretion detection kit has the advantage that pathogenic bacteria or pathogens of bacterial vaginal diseases and trichomonas vaginitis can be used for assisting in the fast judgment of the vaginal disease condition. The invention also discloses a preparation method of the vaginal secretion detection kit.

Description

technical field [0001] The invention relates to a vaginal secretion detection kit and a preparation method thereof, belonging to the field of female vaginosis detection supplies. Background technique [0002] Vaginitis is an inflammation of the vaginal mucosa and submucosal connective tissue, and is a common disease in gynecological clinics. For normal healthy women, due to anatomical and biochemical characteristics, the vagina has a natural defense function against the invasion of pathogens. When the natural defense function of the vagina is destroyed, pathogens are easy to invade and cause vaginal inflammation. There are three types of common vaginitis: bacterial vaginosis (BV / AV), fungal vaginitis (VVC) and trichomonas vaginitis (TV). Among them, female bacterial vaginal infection can be divided into two types, one is caused by anaerobic bacteria and facultative anaerobic bacteria, clinically called bacterial vaginosis (BV), and the other is caused by aerobic bacteria, c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/56C12Q1/37C12Q1/34
CPCC12Q1/56C12Q1/34C12Q1/37C12Q2334/10C12Q2334/52C12Q2337/12C12Q2337/30G01N2333/924G01N2333/942G01N2333/948G01N2333/952
Inventor 眭红燕朱华琳范静彦李必松
Owner GUANGZHOU HONGQI OPTICAL INSTR TECH
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