32 results about "Coagulase" patented technology

A negatively-charged Staphylococcus antigen contains amino acids and a N-acetylated hexosamine as a major carbohydrate component. The antigen is common to many coagulase-negative strains of Staphylococcus, including S. epidermidis, S. haemolyticus, and S. hominis. Staphylococcus strains that carry the antigen include many clinically significant strains of Staphylococcus. The antigen and antibodies to the antigen are useful in kits and assays for diagnosing Staphylococcus infection. Vaccines of the antigen and of whole cells that carry the antigen also are disclosed.

PCT No. PCT / DK95 / 00511 Sec. 371 Date Aug. 14, 1997 Sec. 102(e) Date Aug. 14, 1997 PCT Filed Dec. 20, 1995 PCT Pub. No. WO96 / 19582 PCT Pub. Date Jun. 27, 1996A method of producing a milk clotting enzyme including the steps of (a) fermenting a strain of Rhizomucor miehei or Aspergillus oryzae to form a fermentation product having a glycoslated Rhizomucor miehei aspartic protease and other proteins, and (b) subjecting a quantity of the fermentation product to a deglycosylating treatment to form a coagulant preparation having an at least partly deglycoslated aspartic protease and the other proteins. The at least partly deglycosylated protease has a milk clotting activity that is at least 10% higher than a milk clotting activity of the glycosylated aspartic protease.

The invention discloses a vaginal fluid detection kit, which comprises a reaction device and a color developing agent, wherein the reaction device comprises a reaction hole for detecting beta-glucuronidase, a reaction hole for detecting acetylglucosaminidase, a reaction hole for detecting coagulase and a reaction hole for detecting proline aminopeptidase; when the kit is used for detecting vaginal secretion, the used diluent is normal saline. The vaginal secretion detection kit has the advantage that pathogenic bacteria or pathogens of bacterial vaginal diseases and trichomonas vaginitis can be used for assisting in the fast judgment of the vaginal disease condition. The invention also discloses a preparation method of the vaginal secretion detection kit.

The present invention relates to monoclonal antibodies capable of recognising and binding to the PBP2-a protein and to other proteins having sequences homologous to PBP2-a, including pathogenic species such as the methyciline-resistant Staphylococcus Aureus (MRSA), coagulase-negative Staphilococcus, Staphylococcus sciuri and Enterococcus, and any other bacteria containing the PBP2-a protein or homologous sequences. The invention also relates to the use of the monoclonal antibodies capable of recognising and binding to the PBP2-a protein and to other proteins having sequenceshomologous to PBP2-a in a complementary immunodiagnostic test for detecting resistance to beta-lactam antibiotics.

The invention discloses an eight-joint detection kit for vaginitis. The eight-joint detection kit is characterized by comprising a reaction plate and (2) a diluent, wherein the reaction plate is provided with reaction holes for detecting the following indexes: beta-glucuronidase, coagulase, hydrogen peroxide, neuraminidase, leukocyte esterase, acetamido-beta-glucosidase, proline aminopeptidase andpH. The kit aims to solve the problem that aerobic bacterial vaginitis, bacterial vaginitis, trichomonas vaginitis and mycotic vaginitis cannot be detected at the same time, and meanwhile, the problem that the kit is poor in detection precision is solved.

The invention discloses a joint detection kit for detecting and distinguishing Trichomonas and Candida, which comprises coagulation enzyme activity detection reagent, α-glucosidase activity detection reagent and pH detection reagent; the enzyme substrate used in the coagulation enzyme activity detection reagent The substance is a Gly-Arg dipeptide substrate containing a chromogen or a fluorescent group; the enzyme substrate used in the α-glucosidase activity detection reagent is an α-D-glucoside substrate containing a chromogen or a fluorescent group . In order to improve the color developing effect, it may also include a color developing solution. The invention has the advantages of relatively high sensitivity and specificity, significantly improved performance of reagents compared with the existing similar methods, and can meet the needs of clinical detection.

The invention discloses a vaginal secretion dry chemical analysis liquid quality control material and a preparation method thereof. A corresponding buffer system is prepared first, and then, with the buffer system as a solvent, a plurality of raw materials of a negative quality control material and a positive quality control material are added and dissolved; when different components are added, the next component can be added after the previous component is completely dissolved; in order to ensure the enzyme activity, a liquid negative quality control material and a liquid positive quality control material are prepared in an environment of 4-15 DEG C, and the effect is better; after the preparation is completed, subpackaging and storage are carried out within 24 hours, and quality control can be carried out on 10 items such as coagulase, hydrogen peroxide, lactic acid, oxidase, glucuronidase, N-acetyl hexosaminidase, sialidase, leukocyte esterase, proline aminopeptidase and pH value at the same time, so that the stability is good, the period is long, the use is convenient and fast, complex step of redissolving before the freeze-dried product is used is omitted, and errors caused by redissolving are reduced; and the liquid quality control material has a good practical application value.

The present invention relates to monoclonal antibodies capable of recognising and binding to the PBP2-a protein and to other proteins having sequences homologous to PBP2-a, including pathogenic species such as the methyciline-resistant (MRSA), coagulase-negative, and , and any other bacteria containing the PBP2-a protein or homologous sequences. The invention also relates to the use of the monoclonal antibodies capable of recognising and binding to the PBP2-a protein and to other proteins having sequences homologous to PBP2-a in a complementary immunodiagnostic test for detecting resistance to beta-lactam antibiotics.

The invention discloses a pressure-sensitive autogenous thermogenic plant microbial treatment hot compress patch and a preparation method thereof. The hot compress patch is prepared from blackcurrant,violet herba Violae, radix scrophulariae, radix Angelicae sinensis, radix Paeoniae alba, flos Lonicerae, cactus, vinyl acetate and acrylate. 2-Ethylene Hexyl Ester, 2-Ethyl-4. Functional components obtained by polymerizing methimidazole crosslinking agent, bentonite, corn starch, purified water and absolute ethanol with sufficient amount of saprophytic staphylococcus bacteria, coagulase-negativestaphylococcus bacteria and septococcus luteus bacteria through aqueous monomer copolymerization; The other part is woven fabric made of cotton fibers according to the design of hot compress sticker.The last part is PE plastic sleeve for vacuum packaging. As that pressure-sensitive adhesive of the invention has good adhesion, good skin affinity, spontaneous heat generation, green environmental protection and pollution-free, and obvious moxibustion effect, the pressure-sensitive adhesive is suitable for industrial batch production and self-adhesion, and has pharmaceutical treatment effect andprobiotic microbial health care effect. The pressure-sensitive adhesive of the invention has good adhesion, good skin affinity, self-heat generation, green environmental protection and pollution-free,obvious moxibustion effect, and is suitable for industrial batch production and self-adhesion.

The invention provides a human sepsis pathogen detection kit and a corresponding detection method. The kit provided by the invention comprises primers and fluorescent probes fordetecting acinetobacter baumannii, klebsiella pneumoniae, streptococcus pneumoniae, bacteroides fragilis, staphylococcus aureus, pseudomonas aeruginosa, aeromonas hydrophila, escherichia coli, quail chicken enterococcus, enterobacter cloacae, stenotrophomonas maltophilia, coagulase negative staphylococcus, candida albicans, candida tropicalis, candida glabrata, cytomegalovirus, human herpesvirus type 4, human parvovirus B19, Candida parapsilosis, streptococcus.

The invention provides an in-vitro inflammation model of dairy cow mammary epithelial cells. The in-vitro inflammation model is an injury pathological model of coagulase negative staphylococcus infected dairy cow mammary epithelial cells. According to the invention, cow mammary epithelial cells are taken as a research object, a method for infecting cow mammary epithelial cells with coagulase-negative staphylococcus by taking cow mammary epithelial cells as a carrier is established, an in-vitro inflammation model for simulating bacterial infection of cow mammary cells is established, and proper infection complex number MOI and infection time are determined; and optimizing and determining the detection index of the coagulase-negative staphylococcus infected cow mammary epithelial cells. The model is stable and reliable, and lays a foundation for in-vitro drug screening research on cow mastitis caused by bacteria, especially coagulase-negative staphylococcus, and pathogenic mechanism research on bacteria, especially coagulase-negative staphylococcus.

The invention provides a kit for detecting aerobic bacteria in vaginal discharge, a dried kit comprises a reactor for detecting the enzyme activity of beta-glucuronidase and coagulase and color-developing agent, and a humidified kit comprises beta-glucuronidase substrate solution, coagulase substrate solution and color-developing agent. The invention also provides a preparation method of the kit.The kit in the invention directly identifies the aerobic flora, in particular to the aerobic floras such as staphylococcus aureus, Enterococcus faecalis, streptococcus group B, Escherichia coli and the like which can cause aerobic bacteria vaginalitis in vaginal discharge by detecting the activity of beta-glucuronidase and coagulase. The method has simple, convenient and rapid operation and high accuracy, and is suitable for convention application in clinics especially in hospital outpatient.

The invention provides an AV / BV joint detection kit, which is composed of a reaction device, a sample diluent, a chromogenic agent A and a chromogenic agent B, wherein the reaction device is provided with a hydrogen peroxide reaction hole, a sialidase reaction Well, white blood cell esterase reaction well, β-glucuronidase reaction well and coagulation enzyme reaction well, there are corresponding reaction pads in the reaction well. The invention also provides a preparation method of the detection kit. The detection kit of the present invention can directly identify the aerobic bacteria / Anaerobic bacteria and facultative anaerobic bacteria and other flora, the method is simple, fast, and highly accurate, and is suitable for routine clinical use, especially in outpatient clinics.

Disclosed is a technique for obtaining a coagulogen raw material which can irreversibly inactivate the activity of a coagulase while retaining the function of coagulogen in an LAL reagent, a LAL reagent contaminated by an organism-derived biologically active substance or the like, and which can be used in a reagent. An LAL reagent is heated at a predetermined temperature for a predetermined period of time to deactivate only the activity of an enzyme contained in the LAL reagent irreversibly, wherein such an activity inherent in coagulogen that coagulogen can be hydrolyzed with the activated coagulase and converted to coagulin to induce gelatinization or an agglutination reaction is retained.

The invention provides a detection kit for human sepsis pathogens and a corresponding detection method. The kit of the present invention includes detection of Acinetobacter baumannii, Klebsiella pneumoniae, Streptococcus pneumoniae, Bacteroides fragilis, Staphylococcus aureus, Pseudomonas aeruginosa, Aeromonas hydrophila, Escherichia coli bacteria, Enterococcus gallinarum, Enterobacter cloacae, Stenotrophomonas maltophilia, Coagulase-negative Staphylococcus, Candida albicans, Candida tropicalis, Candida glabrata, Cytomegalovirus, Human herpesvirus type IV , Human parvovirus B19, Candida parapsilosis, Streptococcus primers and fluorescent probes.