8 results about "Coagulase" patented technology

The invention discloses a salt-tolerant staphylococcus warneri strain WB224, and the preservation registration number of the salt-tolerant staphylococcus warneri strain WB224 is CGMCC No: 28260. The invention also discloses two applications of the strain WB224: 1, the strain WB224 is applied to thick broad-bean sauce flavored fermented foods (thick broad-bean sauce, chilli sauce and fermented soya beans); and 2, application in improvement of acid soil. The strain WB224 is obtained from a fermented food Pixian thick broad-bean sauce, the coagulase reaction is negative, the strain has the characteristics of strong stress resistance and high salt resistance, the formation of flavor substances such as esters and alcohols in the thick broad-bean sauce flavor fermented food can be obviously promoted, and the flavor of a product is effectively improved. Meanwhile, the acid soil with the pH value of 4.5-5.0 can be improved to a subacid environment with the pH value of 6.0-6.5; the effect is lasting and stable, and secondary pollution is avoided. The method has dual application values of food fermentation and soil improvement, so that the method has a wide industrial application prospect.

This provides a method for identifying S. aureus and other bacterial species that make up the coagulase-negative staphylococcus group. [Solution] A method for determining the presence or absence of Staphylococcus species in a species-specific manner, wherein the Staphylococcus species is one or more species selected from the group consisting of S. aureus, S. epidermidis, S. capitis, S. caprae, S. hominis, and S. lugdunensis, and comprises a peak detection step of determining whether one or more peaks selected from a group consisting of peaks corresponding to specific m / z values ​​are detected in a mass spectrum obtained using matrix-assisted laser desorption / ionization mass spectrometry on a test sample with respect to S. aureus, and a bacterial species determination step of determining the presence or absence of S. aureus based on the peak detection result of the peak detection step.

The invention relates to the field of modern pharmacy of traditional Chinese medicines, and discloses application of leonurine in preparation of antibacterial products. According to the invention, the in-vitro antibacterial activity of leonurine is detected by an agar dilution method and a broth trace dilution method. The result shows that the leonurine can inhibit the proliferation of a staphylococcus aureus standard strain, and also has an inhibiting effect on most of methicillin-sensitive staphylococcus aureus, methicillin-resistant staphylococcus aureus and methicillin-resistant coagulase-negative staphylococcus which are obtained by clinical separation; meanwhile, the leonurine can inhibit proliferation of a malassezia standard strain and a clinical isolated strain, also has an inhibiting effect on most of ketoconazole drug-resistant candida and ketoconazole dose dependence-sensitive candida obtained through clinical isolation, and can be used for preparing antibacterial pharmaceutical compositions and skin care products.

The invention provides a quality control product for detecting vaginal secretion and a preparation method of the quality control product. The quality control product comprises a negative quality control product and a positive quality control product; the positive quality control product comprises 20-200 mM of a second basic buffer solution, 1-5% of a second enzyme protective agent, 0-0.04% of lactic acid, 0-0.005% of hydrogen peroxide, 0.01-0.1 U / mL of a second leukocyte esterase item, 0.02-0.1 U / mL of sialidase, 0.05-0.5 U / mL of a second N-acetylglucosaminidase item, 50-200 U / mL of a second oxidase item, 2-40 U / mL of a second coagulase item, 50-200 U / mL of beta-glucuronidase, 5-20 U / mL of proline aminopeptidase, 1-5 U / mL of a second alkaline phosphatase item and 0.2-0.2. And 1% of bovine serum albumin.

The invention relates to the field of biological detection, in particular to a detection reagent, coagulase detection test paper and application thereof. The invention provides a coagulase detection reagent. The coagulase detection reagent comprises a soak solution I and a soak solution II, the soaking solution I is prepared from a buffer solution, polyvinylpyrrolidone K90 or K30, glycerol and glycyl-arginyl-4-methoxy-beta-naphthylamine or glycyl-arginine-beta-naphthylamine, and the soaking solution II is prepared from a buffer solution, polyvinylpyrrolidone K90 or K30, glycerol and glycyl-arginyl-4-methoxy-beta-naphthylamine; the soaking solution II is prepared from polyvinylpyrrolidone K90 or K30, peregal, trehalose and dimethylamino cinnamyl aldehyde. The coagulase detection test paper provided by the invention can directly develop color after being dropwise added into a sample, does not need to additionally add a developing solution, and is more convenient and faster and suitable for popularization compared with traditional two-step method test paper.

The present invention provides a primer-probe combination, a kit, and its application for detecting bloodstream infection pathogens. Specifically, the present invention provides a primer-probe combination for detecting bloodstream infection pathogens, the primer-probe group comprising: (a) upstream and downstream primers and probes for detecting coagulase-negative Staphylococci as shown in SEQ ID NOs: 7 to 9; (b) upstream and downstream primers and probes for detecting Escherichia coli as shown in SEQ ID NOs: 1 to 3; (c) upstream and downstream primers and probes for detecting Klebsiella pneumoniae as shown in SEQ ID NOs: 4 to 6; and (d) upstream and downstream primers and probes for detecting Staphylococcus aureus as shown in SEQ ID NOs: 10 to 12. The primer-probe combination of the present invention can be used to achieve rapid, highly sensitive, and highly specific detection of bloodstream infection pathogens.

The application discloses a coagulase Heyndrickx strain CCFM1539 with high spore yield and promoting intestinal calcium and zinc absorption and a postbiotic thereof, and belongs to the technical field of microorganisms. In the application, the absorption amount of zinc and calcium is significantly increased, which is specifically embodied in the following aspects: (1) the coagulase Heyndrickx strain CCFM1539 strain cell component significantly improves the expression level of zinc and calcium transport protein genes of Caco-2 cells; (2) the coagulase Heyndrickx strain CCFM1539 significantly increases the total transport amount of zinc and calcium of a Caco-2 cell monolayer model; and (3) the coagulase Heyndrickx strain CCFM1539 significantly promotes the absorption of zinc and calcium in a mouse body. Therefore, the coagulase Heyndrickx strain CCFM1539 has great application prospect in promoting intestinal zinc and calcium absorption.

The present application relates to the technical field of food processing, and discloses a compound fermentation aid for fermented meat products and application thereof, which comprises a functional bacteria compound, a flavor precursor and enzyme preparation compound package, and adaptive accessories; the functional bacteria compound is composed of lactic acid bacteria, coagulase-negative staphylococcus and yeast bacteria at a ratio of 60-70%:25-35%:5-10% in terms of viable bacterial count, the flavor precursor and enzyme preparation compound package comprises oligopeptide, free amino acid, fatty acid, neutral protease and lipase, and the adaptive accessories comprise protective agent and carrier, fermentation accelerator and antioxidant. The compound fermentation aid can quickly establish an acidic environment to inhibit pathogenic bacteria, directionally regulate the generation of flavor substances to improve flavor stability, improve product color and texture, and shorten the fermentation period, thereby effectively solving the problems of unstable flavor, difficult safety control, large quality fluctuation and long fermentation period in the production of fermented meat products in the prior art.