Kit for detecting aerobic bacteria in vaginal discharge and preparation method thereof

Abstract

The invention provides a kit for detecting aerobic bacteria in vaginal discharge, a dried kit comprises a reactor for detecting the enzyme activity of beta-glucuronidase and coagulase and color-developing agent, and a humidified kit comprises beta-glucuronidase substrate solution, coagulase substrate solution and color-developing agent. The invention also provides a preparation method of the kit.The kit in the invention directly identifies the aerobic flora, in particular to the aerobic floras such as staphylococcus aureus, Enterococcus faecalis, streptococcus group B, Escherichia coli and the like which can cause aerobic bacteria vaginalitis in vaginal discharge by detecting the activity of beta-glucuronidase and coagulase. The method has simple, convenient and rapid operation and high accuracy, and is suitable for convention application in clinics especially in hospital outpatient.

Description

technical field [0001] The invention relates to a kit for detecting microorganisms and a preparation method thereof, in particular to a kit for detecting aerobic flora in vaginal secretions and a preparation method thereof. Background technique [0002] Aerobic vaginitis is a newly discovered vaginitis in recent years, accounting for about 20% of vaginal infections. The main pathogenic bacteria are Group B Streptococcus, Enterococcus faecalis, Staphylococcus aureus and Escherichia coli. At present, the commonly used method for detecting aerobic bacteria in vaginal secretions is the microbial culture method, which is to inoculate the vaginal secretions on a blood plate, separate and identify them after cultivation. This method is complicated, time-consuming, and inconvenient for clinical routine, especially Outpatient use. [0003] Bacterial preformed enzymes are a series of enzymes related to growth metabolism synthesized by bacteria during the growth and reproduction proce...

AI Technical Summary

Problems solved by technology

At present, the preformed enzyme method has been widely used in the identification and detection of microorganisms in samples, especially anaerobic bacteria, such as the detection of Gardnerella vaginalis infection by the activity of sialidase in vaginal secretions, and the detection of G. Protease activity was used to detect vaginal Candida albicans infection, etc., but there was no report on the use of bacterial preformed enzymes to directly identify aerobic flora in vaginal secretions

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