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Coagulase negative staphylococcus selective medium and preparation method thereof

A staphylococcus, selective technology, applied in the field of medical testing, can solve the problem of not providing CNS selective culture, and achieve the effect of reliable detection and high separation rate

Inactive Publication Date: 2013-04-03
孟蕾蕾
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention does not provide any information on the selective cultivation of the CNS

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1, the formula for preparing 1000ml of selective medium contains: 15g of malt extract powder; 150ml of egg white liquid; 5g of sodium chloride; 0.5g of magnesium citrate; 15g of agar; 3.6 g; monopotassium phosphate 0.2 g; add distilled water to 1000ml. Preparation method: Dissolve malt extract powder; egg white liquid; sodium chloride; magnesium citrate; , sterilized twice at low temperature, 45 min each time, and packed after sterilization.

Embodiment 2

[0029] Example 2, the formula for preparing 1000ml of selective medium contains: 15g of malt extract powder; 150ml of egg white liquid; 5g of sodium chloride; 0.5g of magnesium citrate; 15g of agar; 3.6 g; monopotassium phosphate 0.2 g; copper sulfate 5mg; distilled water added to 1000ml. Preparation method: take malt extract powder; egg white liquid; sodium chloride; magnesium citrate; agar; Make up to volume with distilled water, sterilize twice at low temperature, 45 min each time, and pack after sterilization.

Embodiment 3

[0030] Example 3, the formula for preparing 1000ml of selective medium contains: 15g of malt extract powder; 150ml of egg white liquid; 5g of sodium chloride; 0.5g of magnesium citrate; 15g of agar; 3.6 g; monopotassium phosphate 0.2 g; L-cysteine 0.1 g; add distilled water to 1000ml. Preparation method: take malt extract powder; egg white liquid; sodium chloride; magnesium citrate; agar; After uniform filtration, distilled water to volume, low-temperature sterilization twice, 45 min each time, after sterilization, aliquot.

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PUM

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Abstract

The invention discloses a coagulase negative staphylococcus selective medium and a preparation method thereof. The coagulase negative staphylococcus selective medium and the preparation method are used in the field of inspection and susceptibility testing. The coagulase negative staphylococcus selective medium is characterized in that a formula for preparing 1000ml of the selective culture medium comprises the following components: 15g of malt extract, 150ml of egg protein fluid, 5g of sodium chloride, 0.5g of magnesium citrate, 15g of agar, 8-12mg of coetsoidin A, 3.6g of asparagine, 0.2g of monopotassium phosphate and distilled water added to 1000ml. The preparation method of the best optimization formula comprises the following steps of: dissolving the malt extract, the egg protein fluid, sodium chloride, magnesium citrate, the agar, the coetsoidin A, the asparagine, the monopotassium phosphate, L-cysteic acid and copper sulfate to lily decocted liquid, uniformly mixing, metering volume and split charging after sterilization. Compared with the prior art, the method has the characteristics of being reliable to detect and high in separation rate of coagulase negative staphylococcus.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a coagulase-negative staphylococcus selective culture medium and a preparation method thereof. Background technique [0002] Staphylococcus is a relatively common clinical infection. With the widespread use of a large number of cephalosporins, especially the third-generation cephalosporins and other high-efficiency broad-spectrum antibiotics, methicillin-resistant Staphylococcus (MRS) is increasing worldwide. Methicillin-resistant coagulase-negative staphylococci (MRCNS), as a member of the MRS and coagulase-negative staphylococcus (CNS) families, have an increasing detection rate and multi-drug resistance year by year, and have become the main pathogenic bacteria of nosocomial infections one. CNS is a low-virulence opportunistic pathogen with atypical symptoms after infection and multi-drug resistance, which brings certain difficulties to clinical diagnosis and treatment. Efficie...

Claims

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Application Information

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IPC IPC(8): C12Q1/14C12R1/44
Inventor 孟蕾蕾
Owner 孟蕾蕾
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