A method for extracting theaflavins from black tea
A technology for theaflavin and black tea, applied in the field of plant extraction, can solve the problems of low yield of natural theaflavin, use of toxic reagents, low purity and the like, and achieves overcoming the low yield and purity, simple steps and high yield Effect
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Embodiment 1
[0054] The extraction method of embodiment 1 black tea theaflavins
[0055] like figure 1 Shown, the extraction method of black tea theaflavin, the steps are as follows:
[0056] (1) Use Yinghong No. 9 black tea as raw material, pass through a 60-mesh sieve after crushing, add 50% ethanol solution according to the solid-to-liquid ratio (w / v) of 1:10, reflux extraction for 3 times, combine the extracts and rotate to concentrate to ethanol-free;
[0057] (2) Use ethyl acetate to extract the concentrated extract of black tea, the volume ratio of the concentrated extract of black tea to ethyl acetate is 1:1, the number of extractions is 3 times, the ethyl acetate extract is combined and rotated to concentrate to remove ethyl acetate , freeze-dried to obtain the ethyl acetate layer extract;
[0058] (3) Get ethyl acetate layer extract and dissolve with 80% methanol solution (adjust pH value to 3.5 with acetic acid), wash with 80% methanol solution (pH value is 3.5) after removin...
Embodiment 2
[0062] The extraction method of embodiment 2 black tea theaflavins
[0063] like figure 1 Shown, the extraction method of black tea theaflavin, the steps are as follows:
[0064] (1) Choose Jinxuan black tea as raw material, pass through a 60-mesh sieve after crushing, add 50% ethanol solution according to the solid-to-liquid ratio (w / v) of 1:8, reflux and extract 4 times, combine the extracts and concentrate them by rotation to nothing ethanol;
[0065] (2) Use ethyl acetate to extract the concentrated extract of black tea, the volume ratio of the concentrated extract of black tea to ethyl acetate is 1:2, the number of extractions is 2 times, the ethyl acetate extract is combined and rotated to concentrate to remove ethyl acetate , freeze-dried to obtain the ethyl acetate layer extract;
[0066] (3) Get ethyl acetate layer extract and dissolve with 85% methanol solution (adjust pH value to 3.0 with acetic acid), wash with 85% methanol solution (pH value is 3.0) after remov...
Embodiment 3
[0071] Example 3 Determination of the purity of theaflavins in Yinghong No. 9 black tea
[0072] 1 Experimental method
[0073] 1.1 Instruments and reagents
[0074] Instruments: high performance liquid chromatography, ultrasonic cleaner, sand core suction filter, ultrapure water generator, analytical balance (sensitivity 0.0001g), constant temperature water bath, etc.
[0075] Reagents: acetonitrile chromatographically pure; ethyl acetate and acetic acid are of superior grade; standard products: TF2A, TF2B, TF3, TF.
[0076] 1.2 Chromatographic conditions
[0077] Mobile phase: A phase: acetonitrile: ethyl acetate = 7:1 (v: v) B phase: 2% acetic acid
[0078] Flow rate: 0.8mL / min
[0079] Column temperature: 35°C
[0080] Wavelength: UV 280nm
[0081] Column: C18 chromatographic column (particle size 5μm, 250mm×4.6mm)
[0082] Injection volume: 10μL
[0083] Elution: Gradient elution, the gradient elution program is shown in Table 1 below:
[0084] Table 1
[0085] ...
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