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A kind of Lactobacillus brucelli and application thereof
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A technology of Lactobacillus brucei and Lactobacillus brucei, applied in the direction of bacteria, microorganisms, microorganisms, etc., can solve the problems of low transformation yield and low product concentration, and achieve the effects of rapid transformation, good biological safety, and easy amplification
Active Publication Date: 2019-08-06
ZHEJIANG UNIV OF TECH
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Problems solved by technology
[0005] However, the synthesis of these high-value organic acids through microbial fermentation or transformation often has problems such as strain safety, high efficiency, low conversion yield, and low product concentration.
Method used
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Examples
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Effect test
Embodiment 1
[0025] The invention isolates and screens strains from traditional kimchi. Take a certain amount of kimchi juice, dilute it in sterile water, and put it on a solid medium containing calcium carbonate (composition: glucose 20 g, peptone 10 g, diamine hydrogen citrate 2 g, anhydrous sodium acetate 5 g, manganese sulfate 0.25 g, magnesium sulfate 0.58 g, dipotassium hydrogen phosphate 2 g, yeast extract 5 g, beef extract 10 g, Tween 80 1 g, agar 20 g, calcium carbonate 30 g, deionized water 1 L, pH natural) Primary screening, temperature 30 ℃, static culture 24~72h.
[0026] Select a single colony negative for hydrogen peroxide and inoculate it in a liquid medium (composition: 20 g glucose, 10 g peptone, 2 g diamine hydrogen citrate, 5 g anhydrous sodium acetate, 0.25 g manganese sulfate, 0.58 g magnesium sulfate, phosphoric acid Dipotassium hydrogen dipotassium 2 g, yeast extract 5 g, beef extract 10 g, Tween 80 1 g, deionized water 1 L, pH value natural), 30 ℃ static culture f...
Embodiment 2
[0030] With Lactobacillus Brucella described in the present invention ( Lactobacillus buchneri ) GBS3 strain is the starting strain, in 1 L liquid medium (composition: glucose 20 g, peptone 10 g, diamine hydrogen citrate 2 g, anhydrous sodium acetate 5 g, manganese sulfate 0.25 g, magnesium sulfate 0.58 g, phosphoric acid Dipotassium hydrogen dipotassium 2 g, yeast extract 5 g, beef extract 10 g, Tween 80 1 g, deionized water 1L, pH value natural) for static fermentation, temperature controlled at 25~35°C, fermentation for 24 hours, obtained Fermentation broth containing lactic acid and phenyllactic acid; detected and analyzed by high performance liquid chromatography, the concentration of lactic acid in the obtained fermentation broth was 0.5 g / L, and the final concentration of phenyllactic acid was 1 mg / L.
Embodiment 3
[0032] With Lactobacillus Brucella described in the present invention ( Lactobacillus buchneri ) The GBS3 strain was the starting strain, and it was fermented statically in 1 L of liquid culture medium (the composition was the same as in Example 2), and the temperature was controlled at 25-35°C, and the fermentation was carried out for 72 hours to obtain a fermentation liquid containing lactic acid and phenyllactic acid; Detection and analysis by liquid chromatography showed that the concentration of lactic acid in the fermented broth was 1.8 g / L, and the final concentration of phenyllactic acid was 13 mg / L.
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Abstract
The present invention provides a strain of Lactobacillus brucei and its application in fermentation and transformation to synthesize high-value organic acids, and the strain of Lactobacillus brucei is named ( Lactobacillus buchneri ) GBS3, deposited in China Center for Type Culture Collection, deposit number: CCTCC No.M 2016215. Taking the bacterial strain provided by the present invention as the starting strain, phenyllactic acid and lactic acid are simultaneously synthesized through microbial fermentation and metabolism; the bacterial cell of the bacterial strain provided by the present invention is used as a whole-cell catalyst, and phenylpyruvate and glucose are used as substrates, and transformed by microorganisms Synthesis of phenyllactic acid. The strain provided by the invention is isolated from traditional fermented food kimchi, and has good biological safety, mild conversion reaction conditions and high conversion rate; the final concentration of lactic acid in the fermentation broth can reach 1.8 g / L, and the final concentration of phenyllactic acid is 13 mg / L ; The concentration of phenyllactic acid in the conversion solution can reach 12 g / L, which has a good industrial application prospect.
Description
technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a microbial strain of Lactobacillus brauchneri and its application in synchronous fermentation synthesis and conversion synthesis of high-value organic acids. Background technique [0002] Organic acids have important applications in many fields such as chemical industry, materials, agriculture, medicine and food. The use of microbial fermentation or microbial conversion to synthesize organic acids has the advantages of mild preparation conditions, environmental friendliness, and relatively simple synthesis routes, and is an important direction in the field of biochemical engineering. [0003] Both lactic acid and phenyllactic acid are very important high-value organic acids. As a carboxylic acid with a hydroxyl group, lactic acid has a wide range of uses, such as clinically used in disinfectants, surgical sutures, pharmaceutical preparations, in the fie...
Claims
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