Method for extracting tissue RNA by tissue grinding

Abstract

The invention discloses a method for extracting tissue RNA by tissue grinding, and belongs to the technical field of molecular biology. The method comprises the following steps: (1) burning the tip of an RNA enzyme-free disposable plastic pipette head with flame of an alcohol lamp to soften the tip to form a round bump; (2) putting a tissue for extracting RNA into an RNA enzyme-free disposable plastic EP pipe; and (3) grinding the pipette head prepared in the step (1) in the EP pipe in the step (2). According to the method for extracting tissue RNA by tissue grinding, a burnt RNA enzyme-free disposable plastic pipette head is adopted to grind a tissue with the RNA enzyme-free disposable plastic EP pipe. Compared with a traditional glass or ceramic homogenizer, the method has the advantages that the RNA extraction operation is more time-saving, simple and convenient, materials are wide in source, and the cost is relatively low. The grinding pipette head has a relatively small size, is especially suitable for extracting RNA of micro-tissues, and can be used for grinding sufficiently, so that the RNA yield and quality can be improved.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a tissue grinding method for extracting tissue RNA. Background technique [0002] In the traditional method of extracting tissue RNA, glass or ceramic grinding rods are mostly used to grind the tissue, but untreated grinding rods will be contaminated by RNase (which can catalyze the hydrolysis of RNA). Therefore, it is necessary to soak the grinding rod in acid overnight, rinse it, soak it in DEPC water for about 8 hours, dry it at 37°C, wrap it in tin foil and dry bake it several times. This preparation process is more complicated and time-consuming. . In addition, since the tissue pieces used for RNA extraction are often small and the homogenizer is large, the liquid loss is large during the grinding process, and the grinding is not thorough enough, which will affect the yield and quality of RNA. Contents of the invention [0003] The purpose of the present invent...

AI Technical Summary

Problems solved by technology

[0002] In the traditional method of extracting tissue RNA, glass or ceramic grinding rods are mostly used to grind the tissue, but untreated grinding rods will be contaminated by RNase (which can catalyze the hydrolysis of RNA)

Therefore, it is necessary to soak the grinding rod in acid overnight, rinse it, soak it in DEPC water for about 8 hours, dry it at 37°C, wrap it in tin foil and dry bake it several times. This preparation process is more complicated and time-consuming.

In addition, since the tissue pieces used for RNA extraction are often small, and the homogenizer is large, the liquid loss is large during the grinding process, and the grinding is not thorough enough, which will affect the yield and quality of RNA