Method for inducing tobacco calluses by using Lingfasu
The technology of callus and lingfasu is applied in the field of plant bioengineering tissue culture, and can solve the problems of complicated and labor-intensive preparation of culture medium, decreased ability of proliferation and re-differentiation, and low actual yield of callus, etc. Improve overall work efficiency, slight browning and hardening, and reduce culture costs
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0019] A method for inducing tobacco callus with linguine, comprising the following steps:
[0020] Step 1. Preparation of induction medium: use MS as the basic medium, add 0.05 mg / L of Lingfalin, adjust the pH of the medium to 5.8-6.0, and sterilize it for later use;
[0021] Step 2, explant preparation: take the sterile tobacco plants obtained from tobacco seeds, under aseptic conditions, cut the tobacco leaves into square slices as explants, and set aside, the square slices can be cut into about 5mm×5mm in size ;
[0022] Step 3, inoculation and induction culture: insert the tobacco leaf explants obtained in step 2 into the induction medium of step 1 under aseptic conditions, and culture for 10-20 days.
Embodiment 2
[0024] A method for inducing tobacco callus with linguine, comprising the following steps:
[0025] Step 1. Preparation of induction medium: use MS as the basic medium, add 1.5 mg / L of lingfalin, adjust the pH of the medium to 5.8-6.0, and sterilize it for later use;
[0026] Step 2, explant preparation: take the sterile tobacco plant obtained from tobacco seeds, when it grows to 5 or 6 leaves, take the third leaf from top to bottom, and cut it into square pieces under aseptic conditions As an explant, spare, the square piece can be cut into a size of about 5mm×5mm;
[0027] Step 3, inoculation and induction culture: insert the tobacco leaf explants obtained in step 2 into the induction medium of step 1 under aseptic conditions, and culture for 10-20 days.
[0028] When the leaves of sterile tobacco plants grow to 5 or 6 leaves, the third leaf from top to bottom is a fully mature and robust functional leaf with strong cell metabolism and strong vitality. Compared with other t...
Embodiment 3
[0030] A method for inducing tobacco callus with linguine, comprising the following steps:
[0031] Step 1. Preparation of induction medium: use MS as the basic medium, add 2.0 mg / L of lingfalin, adjust the pH of the medium to 5.8-6.0, and sterilize it for later use;
[0032] Step 2, explant preparation: take the sterile tobacco plant obtained from tobacco seeds, when it grows to 5 or 6 leaves, take the third leaf from top to bottom, and cut off 2 mm above the petiole under aseptic conditions and the blade part 2mm below the tip, and leave the middle part of the blade, and then cut it into 5mm×5mm square slices along the two sides of the main vein as explants for future use. It should be noted that the aforementioned section along the main vein refers to The edge leaf parts on the left and right sides of the leaves are discarded, only the leaf parts on both sides of the main vein;
[0033] Step 3, inoculation and induction culture: insert the tobacco leaf explants obtained in...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More