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A cell scratch test cover

A technique of cell scratching and experimentation, which is applied in the direction of biomass post-processing, bioreactor/fermenter for specific purposes, biochemical equipment and methods, etc. It can solve the problem that the width of the traces drawn can not be completely uniform, and the effect of comparison experiments cannot be achieved. It is very precise, and there are problems such as method errors, so as to achieve the effect of convenient and simple production, low cost and strong identity

Active Publication Date: 2019-03-05
CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, conventional cell scratch experiments use the tip of the pipette to compare the ruler to complete the scratch of the cell. The pipette head must be vertical and cannot be tilted. Can not be completely unified, the effect of the comparative experiment cannot be very accurate

Method used

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Experimental program
Comparison scheme
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Embodiment 1

[0016] refer to Figure 1 to Figure 5 The shape and structure of a cell scratch experiment cover and its use method. The specification of the scratch cover 1 is: the maximum length is equal to the diameter of the circular arc processed at the two ends of the long side of the scratch cover 1 cuboid, and the diameter ϕ=100mm; The trace cover 1 has two slide rail grooves 13 parallel to each other, and is suitable for cell culture dishes with a diameter of ϕ100mm. Use a scratching needle 4 with a diameter of ϕ=2mm.

Embodiment 2

[0018] refer to Figure 1 to Figure 5 The shape and structure of a cell scratch experiment cover and its use method. The specification of the scratch cover 1 is: the maximum length is equal to the diameter of the circular arc processed at the two ends of the long side of the scratch cover 1 cuboid, and the diameter ϕ=60mm; The trace cover 1 has two slide rail grooves 13 parallel to each other, and is suitable for cell culture dishes with a diameter of ϕ60mm. Scratch needle 4 with diameter ϕ=1.5mm.

Embodiment 3

[0020] refer to Figure 1 to Figure 5 The shape and structure of a cell scratch experiment cover and its use method. The specification of the scratch cover 1 is: the maximum length is equal to the diameter of the circular arc processed at both ends of the long side of the scratch cover 1 cuboid, and the diameter ϕ=35mm; The trace cover 1 has a sliding rail groove 13 parallel to each other, which is suitable for cell culture dishes with a diameter of ϕ35mm. Scratch needle 4 with diameter ϕ=1mm.

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Abstract

The invention provides a cell scratch test cover. The cell scratch test cover is characterized by mainly comprising a main cover and a movable clamping cover. The scratch cover is a bridge-like cover of a rectangular solid shape, no cover edge is arranged on the two longer sides of the rectangular solid, and circular cover edges are arranged at both ends of the longer sides of the rectangular solid to be buckled on the circular outer side wall of a cell culture dish. The main cover comprises the cover edge at one end of the scratch cover and the rectangular solid cover, and the cover edge at the other end of the scratch cover is the independently separated movable clamping cover. The end surface of the main cover of the scratch cover and the movable clamping cover are tightly attached to each other under the effect of an elastic tensioning device in case of no external force. The cell scratch test cover has the technical characteristics of convenient and simple manufacturing, low cost, high scratch identity, more precise contrast experiment data and simple and convenient operation.

Description

technical field [0001] The invention relates to a small device for cell experiments, in terms of use, it is a cell scratch cover for cell growth and migration experiments in bioengineering and medicine. technical background [0002] Tumor tissue, uncontrolled proliferation of cancer tissue, abnormal differentiation of tumor cells, and the ability of tumor cells to invade and metastasize are the most basic biological characteristics of malignant tumors, and invasion and metastasis are the main reasons why malignant tumors threaten the health and even life of patients. Therefore, it is of great significance to study the law of tumor invasion and metastasis and its mechanism for the prevention and treatment of malignant tumors. The cell scratch method is one of the methods to measure the motility characteristics of tumor cells. It draws on the uncontrolled proliferation of tumor tissue, abnormal differentiation of tumor cells, and the ability of tumor and cancer cells to invad...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00
CPCC12M23/10C12M23/38C12M41/46
Inventor 廖晓玲徐文峰杨晓玲
Owner CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY