Cd<2+> one-step detection preformed reagent and method

A reagent and solvent technology, applied in the field of prefabricated reagents for one-step detection of Cd2+, can solve the problems of weak specificity and complicated steps, and achieve the effects of low detection cost, high sensitivity and strong method feasibility.

Inactive Publication Date: 2017-03-22
NINGDE NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The object of the invention is to provide a one-step detection Cd 2+ Prefabricated reagents and methods, only one step operation can be used to remove colorless Cd in water samples 2+ Combining with the chromogenic agent to form an orange complex, thus solving the technical problems of the existing rapid detection technology such as cumbersome steps and weak specificity

Method used

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  • Cd&lt;2+&gt; one-step detection preformed reagent and method
  • Cd&lt;2+&gt; one-step detection preformed reagent and method
  • Cd&lt;2+&gt; one-step detection preformed reagent and method

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0032] Specific implementation mode 1: The one-step detection of Cd provided by this implementation mode 2+ The configuration steps and packaging of prefabricated reagents are as follows:

[0033] (1) Weigh 11.2 mg of Victoria Blue B and place it in a centrifuge tube with a cover.

[0034] (2) Add 1000 μL, 0.2 mol / L potassium hydroxide ethanol solution to step (1), and mix well.

[0035] (3) Add 2500 μL, 10% (v / v) nonionic surfactant Triton X-114 to step (2), and mix well.

[0036] (4) Add 666 μL, 3 g / L sodium tartrate solution to step (3), and mix well.

[0037] (5) Add 666 μL, 3 g / L potassium fluoride solution to step (4), and mix well.

[0038] (6) Add 666 μL, 3 g / L thiourea solution to step (5), and mix well.

[0039] (7) Add 2000 μL, 30% (w / v) potassium hydroxide solution to step (6), and mix well.

[0040] (8) Add 3054.6 μL, 50% (w / v) citric acid solution to step (7), and mix well.

[0041] (9) Add 23.54mL solvent to step (8). The solvent is mixed with methanol, et...

specific Embodiment approach 2

[0047] Specific embodiment two: this embodiment is different from specific embodiment one, and the method is as follows:

[0048] (1) Weigh 11.2 mg of p-nitrobenzenediazoaminoazobenzene into a centrifuge tube with a cover.

[0049] (2) Add 1500 μL, 0.3 mol / L sodium hydroxide ethanol solution to step (1), and mix well.

[0050] (3) Add 1000 μL, 20% (v / v) nonionic surfactant Tween 80 to step (2), and mix well.

[0051] (4) Add 333 μL, 6 g / L potassium tartrate solution to step (3), and mix well.

[0052] (5) Add 333 μL, 6 g / L potassium fluoride solution to step (4), and mix well.

[0053] (6) Add 333 μL, 6 g / L thiourea solution to step (5), and mix well.

[0054] (7) Add 3000 μL, 25% (w / v) potassium hydroxide solution to step (6), and mix well.

[0055] (8) Add 3818 μL, 40% (w / v) ammonium citrate solution to step (7), and mix well.

[0056] (9) Add 23.77mL of solvent to step (8), the solvent is mixed with ethanol and pure water at a volume ratio of 1:1, and one-step detectio...

specific Embodiment approach 3

[0060] Specific embodiment three: this embodiment is different from specific embodiment one, and the method is as follows:

[0061] (1) 11.2 mg of p-nitrobenzenediazoaminoazobenzene was placed in a centrifuge tube with a cover.

[0062] (2) Add 1500 μL, 0.2 mol / L potassium hydroxide ethanol solution to step (1), and mix well.

[0063] (3) Add 1500 μL, 10% (v / v) nonionic surfactant Triton X-100 to step (2), and mix well.

[0064] (4) Add 166 μL, 12 g / L potassium tartrate solution to step (3), and mix well.

[0065] (5) Add 166 μL, 12 g / L sodium fluoride solution to step (4), and mix well.

[0066] (6) Add 166 μL, 12 g / L thiourea solution to step (5), and mix well.

[0067] (7) Add 1500 μL, 50% (w / v) sodium hydroxide solution to step (6), and mix well.

[0068] (8) Add 7636 μL, 20% (w / v) sodium citrate solution to step (7), and mix well.

[0069] (9) Add 21.46mL of pure water to step (8), and get a one-step detection of Cd after mixing 2+ A total of 34.09 mL of pre-made re...

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Abstract

The invention discloses a Cd<2+> one-step detection preformed reagent and method. The Cd<2+> one-step detection preformed reagent is prepared from a color developing agent, a nonionic surfactant, tartrate, a fluoride, thiourea, a hydroxide, citric acid or a citrate, and a solvent through mixing. The detection method comprises carrying out seal package on the Cd<2+> one-step detection preformed reagent through a glass sample injection bottle or a plastic centrifuge tube with good transmission of light, carrying out preservation in a dark place, and in use, adding a water sample into the Cd<2+> one-step detection preformed reagent and carrying out Cd<2+> color development after a reaction for 3-5min, or comprises packaging the Cd<2+> one-step detection preformed reagent through a dark reagent bottle, carrying out preservation in dark place, and in use, transferring 0.1mL of the Cd<2+> one-step detection preformed reagent into 3.0mL of a water sample through a pipette, a pipettor or a graduated pipette and carrying out Cd<2+> color development after a reaction for 3-5min. The Cd<2+> one-step detection preformed reagent has the characteristics of simple operation, rapid detection, sensitive detection, low cost, safety and easy carrying. Through combined use of a standard colourimetric card or a portable spectrophotometer, content can be determined.

Description

technical field [0001] The invention relates to a one-step detection of Cd 2+ The prefabricated reagent and its one-step detection of Cd 2+ Methods. Background technique [0002] With the rapid development of industry, some heavy metals, organic pollutants or excessive waste water and waste enter the water environment, causing pollution and harm to the ecological environment and aquatic animals and plants. After the environment is polluted by cadmium, cadmium can be enriched in organisms and enter the human body through the food chain to cause chronic poisoning. After cadmium is absorbed by the human body, it forms cadmium-sulfur protein in the body, which selectively accumulates in the liver and kidney. The kidney can absorb nearly 1 / 3 of the cadmium in the body, and is the "target organ" of cadmium poisoning. [0003] About Cd 2+ The limit is stipulated in the national standard "Drinking Water Hygienic Standard GB / T 5749-2006" ≤0.005mg / L; "Fishery Water Quality Standar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/22G01N21/31G01N21/78
CPCG01N31/22G01N21/31G01N21/78
Inventor 王昌捷叶旸旸柯栋贤阮少江刘春雷
Owner NINGDE NORMAL UNIV
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