Application diluent of enzyme-labeled antibody and preparation method thereof

A technology for enzyme-labeled antibodies and diluents, which is applied to measuring devices, instruments, scientific instruments, etc., can solve problems such as pollution and decreased titer of enzyme-labeled antibodies, and achieve the effect of preventing inactivation

Inactive Publication Date: 2018-05-29
河南省医药科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides an enzyme-labeled antibody application diluent and its preparation method, which solves the problem that the titer of the enzyme-labeled antibody will decrease after being diluted with the existing application diluent, and even cause pollution

Method used

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  • Application diluent of enzyme-labeled antibody and preparation method thereof
  • Application diluent of enzyme-labeled antibody and preparation method thereof
  • Application diluent of enzyme-labeled antibody and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] An enzyme-labeled antibody application diluent, which is prepared by mixing the base solution and calf serum at a volume ratio of 3:1, and each liter of the base solution is prepared from the following components: 8.0g of NaCl, 2.0g of Na 2 HPO 4 12H 2 O, 0.25g of NaH 2 PO 4 2H 2 O, 0.1g of KCl, 2.0g of MgCl 2 2H 2 O, 10.8ml of 25% Tween-20, 36ml of glycerin, 6.75ml of 10g / 100ml sodium sorbate, 2.7ml of 10g / 100ml thimerosal, 4.0ml of gentamicin sulfate injection, 1.0g of gelatin, the balance for distilled water. Concretely prepare according to the following steps:

[0033] Step 1, preparing 25% Tween-20: using distilled water as a solvent to prepare Tween-20 with a volume fraction of 25%;

[0034] Prepare 10g / 100ml sodium sorbate: prepare sodium sorbate with a concentration of 10g / 100ml using ethanol as a solvent;

[0035] Preparation of 10g / 100ml thimerosal: use distilled water as solvent to prepare thimerosal with a concentration of 10g / 100ml;

[0036]Step 2...

Embodiment 2

[0041] An enzyme-labeled antibody application diluent, which is prepared by mixing the base solution and calf serum at a volume ratio of 3.5:1, and each liter of the base solution is prepared from the following components: 8.5g of NaCl, 2.0g of Na 2 HPO 4 12H 2 O, 0.25g of NaH 2 PO 4 2H 2 O, 0.05g of KCl, 1.5g of MgCl 2 2H 2 0. 25% Tween-20 of 10ml, glycerol of 30ml, 10g / 100ml sodium sorbate of 6ml, 10g / 100ml thimerosal of 2ml, 3.5ml gentamycin sulfate injection, 1.0g of gelatin, surplus is distilled water. Concretely prepare according to the following steps:

[0042] Step 1, preparing 25% Tween-20: using distilled water as a solvent to prepare Tween-20 with a volume fraction of 25%;

[0043] Prepare 10g / 100ml sodium sorbate: prepare sodium sorbate with a concentration of 10g / 100ml using ethanol as a solvent;

[0044] Preparation of 10g / 100ml thimerosal: use distilled water as solvent to prepare thimerosal with a concentration of 10g / 100ml;

[0045] Step 2, each liter...

Embodiment 3

[0050] An enzyme-labeled antibody application diluent, which is prepared by mixing the base solution and calf serum at a volume ratio of 4:1, and each liter of the base solution is prepared from the following components: 9.0g of NaCl, 2.0g of Na 2 HPO 4 12H 2 O, 0.25g of NaH 2 PO 4 2H 2 O, 0.2g of KCl, 2.5g of MgCl 2 2H 2 0, 12ml of 25% Tween-20, 38ml of glycerin, 8ml of 10g / 100ml sodium sorbate, 3ml of 10g / 100ml thimerosal, 4.5ml of gentamicin sulfate injection, 1.2g of gelatin, the balance is distilled water. Concretely prepare according to the following steps:

[0051] Step 1, preparing 25% Tween-20: using distilled water as a solvent to prepare Tween-20 with a volume fraction of 25%;

[0052] Prepare 10g / 100ml sodium sorbate: prepare sodium sorbate with a concentration of 10g / 100ml using ethanol as a solvent;

[0053] Preparation of 10g / 100ml thimerosal: use distilled water as solvent to prepare thimerosal with a concentration of 10g / 100ml;

[0054] Step 2, each l...

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Abstract

The invention discloses an enzyme-labeled antibody application diluent, which is prepared by mixing a base solution and calf serum at a volume ratio of 3-4:1, and each liter of the base solution is prepared from the following components: 8.0-9.0g NaCl, 2.0g of Na2HPO4 12H2O, 0.25g of NaH2PO4 2H2O, 0.05-0.20g of KCl, 1.5-2.5g of MgCl2 2H2O, 10-12ml of 25% Tween-20, 30-38ml of glycerol, 6-8ml of 10g / 100ml sodium sorbate, 2-3ml of 10g / 100ml thimerosal, 3.5-4.5ml of gentamicin sulfate injection, 0.8-1.2g of gelatin, and distilled water as the balance. The enzyme-labeled antibody diluent of the present invention can prevent the inactivation of the enzyme-labeled antibody and inhibit the growth of microorganisms such as bacteria and fungi. After diluting the concentrated enzyme-labeled antibody with the enzyme-labeled antibody application diluent, the enzyme-labeled antibody can be stored at 2-8°C for half a year.

Description

technical field [0001] The invention belongs to the technical field of biological reagents, and in particular relates to an enzyme-labeled antibody application diluent and a preparation method thereof. Background technique [0002] Prepared or purchased enzyme-labeled antibodies are generally stored in a concentrated form and frozen (-20°C), and then diluted with a diluent hundreds to tens of thousands of times before being used in diagnostic reagents or experiments. The diluted enzyme-labeled antibody can only be used within 1-3 days at 2-8°C, otherwise the enzyme-labeled antibody cannot be used again due to reduced titer and contamination. The stability of enzyme-labeled antibodies is a necessary condition for the preparation of diagnostic reagents and experimental applications. Therefore, it is necessary to study and prepare diluents that keep enzyme-labeled antibodies stable for a long time (6 months) in the application solution. And the laboratory maintains the stabili...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/535
CPCG01N33/535
Inventor 杨小昂
Owner 河南省医药科学研究院
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