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Kit for in vitro diagnosis of liver cancer and detection method thereof

An in vitro diagnosis and kit technology, applied in measuring devices, instruments, scientific instruments, etc., can solve problems affecting the early detection and treatment of liver cancer, and achieve the effect of strong accuracy and high sensitivity

Active Publication Date: 2018-01-16
江西三惠生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no rapid and efficient diagnostic kit for liver cancer on the market, which seriously affects the early detection and treatment of liver cancer

Method used

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  • Kit for in vitro diagnosis of liver cancer and detection method thereof
  • Kit for in vitro diagnosis of liver cancer and detection method thereof
  • Kit for in vitro diagnosis of liver cancer and detection method thereof

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Embodiment Construction

[0055] The kit for in vitro diagnosis of liver cancer of the present invention will be described in detail below in conjunction with the accompanying drawings and specific examples.

[0056] Kit for in vitro diagnosis of liver cancer, including capture antibody, capture antibody blocking buffer, standard, labeled antibody, washing solution and chromogenic solution, the labeled antibody is selected from HRP labeled secondary antibody; the captured antibody is a proline peptide Monoclonal antibody produced by enzyme; its sensitivity and accuracy are above 70%.

[0057] The capture antibody is a monoclonal antibody obtained by cloning proline peptidase into a eukaryotic expression vector, expressing the protein in mammalian cells, obtaining the corresponding antigen after purification, and immunizing the antigen with the mammal.

[0058] The preparation method of the capture antibody comprises the following steps:

[0059] (1) Antigen preparation: the proline peptidase gene was ...

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Abstract

The invention discloses a kit for in vitro diagnosis of liver cancer. The kit comprises a capture antibody and a detection antibody, wherein the capture antibody is a monoclonal antibody prepared from PLD (prolidase), and the detection antibody is a polyclonal antibody prepared from prolidase. The kit has the advantage that sensitivity and accuracy are high and reach 70% or more.

Description

technical field [0001] The invention relates to the field of kits for cancer diagnosis and detection, in particular to a kit for in vitro diagnosis of liver cancer and a detection method thereof. Background technique [0002] According to the 2015 WHO report, the incidence of liver cancer in China ranks first in the world. Globally, there are 782,000 new cases of liver cancer and 745,000 deaths. Among them, the number of new cases and deaths in China accounted for about 50%; that is to say, there are 400,000 new liver cancer patients waiting to be diagnosed in my country every year. In addition, there are still a large number of patients with liver cirrhosis who need to be differentially diagnosed with liver cancer. At present, there are about 20 million chronic hepatitis B patients in China, and nearly 25% to 30% of chronic hepatitis B patients can develop liver cirrhosis. From this, it can be estimated that there are about 6 million liver cirrhosis patients who need diff...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/531
CPCG01N33/531G01N33/57438
Inventor 余跃飞李其云万以叶
Owner 江西三惠生物科技有限公司