Bletilla striata tissue culture seedling transplantation and acclimation technology

Abstract

The invention discloses a bletilla striata tissue culture seedling transplantation and acclimation technology, which comprises the following steps: 1, preparing sand soil, the sand soil being one of yellow sand soil and black sand soil, frying-heating the sand soil to more than 80 DEG C by virtue of a caldron to evaporate moisture in the sand soil and kill parasite ova, cooling the sand soil to room temperature, and screening the sand soil once with a fine screen for later use; 2, spreading the prepared sand soil into bletilla striata tissue culture seedling bottles which are about 5cm high, the spread sand soil having a thickness capable of just covering the roots of seedlings; 3, placing the seedling bottles in step 2 in a tissue culture room to grow for 15 to 30d, and keeping the room temperature of 20 to 30 DEG C and the humidity of about 70 percent. According to the technology, the tissue culture seedlings are not required to be cleaned in an acclimation process, so that the roots of the seedlings are prevented from damage, meanwhile, manpower, chemicals and time are greatly saved, and complex steps for tissue culture seedling acclimation are simplified.

Description

technical field [0001] The invention relates to the technical field of transplanting and domesticating bletilla striata seedlings, in particular to a technique for transplanting and domesticating bletilla striata tissue-cultured seedlings. Background technique [0002] Before the existing Bletilla striata tissue-cultured seedlings are transplanted into the soil, the medium at the root of the tissue-cultured seedlings needs to be washed with water, disinfected, sprayed with a rooting agent, and then put into the soil to wait for the seedlings to grow new roots before being domesticated. , It takes a lot of labor and takes a long time. When cleaning the seedlings, the roots of the seedlings will be damaged, resulting in a low survival rate of the seedlings during the transplanting process, with a maximum of about 70%. For this reason, we propose a kind of bletilla striata tissue culture seedling transplanting domestication technique. Contents of the invention [0003] Based...

AI Technical Summary

Problems solved by technology

[0002] Before the existing Bletilla striata tissue-cultured seedlings are transplanted into the soil, the medium at the root of the tissue-cultured seedlings needs to be washed with water, disinfected, sprayed with a rooting agent, and then put into the soil to wait for the seedlings to grow new roots before being domesticated. , it takes a lot of labor and takes a long time. When cleaning the seedlings, the roots of the seedlings will be damaged, resulting in a low survival rate of the seedlings during the transplanting process, with a maximum of about 70%.