A driving device for cooling and reheating working medium and its control method for vitrification preservation
A driving device and vitrification technology, which is used in household refrigeration devices, preservation of human or animal bodies, lighting and heating equipment, etc. It can solve the problems of large influence of accidental factors and limited heat exchange efficiency, and achieve good process stability. and consistency, improve stability and consistency, and achieve the effect of large-scale adjustment
Active Publication Date: 2020-05-12
UNIV OF ELECTRONICS SCI & TECH OF CHINA
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[0008] In view of the above-mentioned problems or deficiencies, in order to solve the problems of limited heat exchange efficiency and large influence of operation accidental factors in vitrification preservation, the present invention provides a cooling and reheating working medium driving device and its control system for vitrification preservation. method
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[0033] The present invention will be further described in detail below in conjunction with the embodiments and the accompanying drawings.
[0034] Such as image 3 As shown, a schematic diagram of a cooling and reheating working medium drive device and control method for vitrification preservation, including 1 high-pressure inert gas input port, 2 pressure regulating valve, 3 ball valve, 4 cooling and rewarming working medium container, 5 cooling and rewarming Working medium, 6 sample sections, 7 liquid storage tanks, 8 conduits, 9 thermocouples, 10 data acquisition cards, and 11 computers.
[0035] The high-pressure inert gas used in this embodiment is nitrogen with a pressure of 0.1 MPa and above.
[0036] The inner wall of the conduit 8 is made of polytetrafluoroethylene, which can withstand a low temperature of -200°C, and is wrapped with a heat insulating material, and the heat insulating material is made of foam.
[0037] The sample section 6 stores samples or their ca...
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The invention relates to the field of cooling / rewarming in vitrification preservation of biological materials, in particular to a driving device for cooling and reheating working medium for vitrification preservation and a control method thereof. The device includes a high-pressure inert gas input end, a pressure regulating valve, a ball valve, a cooling and reheating working medium container, a sample section and a liquid storage tank, which are connected in series through conduits. The invention uses high-pressure inert gas as a drive to drive the cooling and reheating working medium in the cooling and reheating container to move through the sample section at high speed and act on the sample or its carrier, thereby realizing the cooling and retemperature of the sample. The present invention uses high-pressure inert gas as the driving force to drive cooling and reheating working medium to achieve vitrification preservation of samples, changing the existing passive mode of action to active, greatly improving the stability and consistency of low-temperature preservation; through the pressure regulating valve To adjust the driving pressure, a wide range of adjustments to the cooling and reheating rate of the sample can be realized.
Description
technical field [0001] The invention relates to the field of cooling / rewarming in vitrification preservation of biological materials, in particular to a driving device for cooling and reheating working medium for vitrification preservation and a control method thereof. Background technique [0002] Low temperature preservation is currently the only effective long-term preservation method for many biological materials, and it plays an irreplaceable role in many fields of national economy and people's livelihood. In the field of clinical medicine, more and more diseases can be cured through cell and tissue transplantation, and large-scale cryopreservation is an important means to solve the problem of clinical supply of these important biological materials. Biomaterials preserved at low temperature need to experience a leap from room temperature to low temperature, and the growth of ice crystals in this process is the biggest obstacle to successful preservation. Vitrification ...
Claims
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IPC IPC(8): A01N1/02A01N1/00F25D3/10F25D29/00
CPCA01N1/00A01N1/0257F25D3/10F25D29/005
Inventor 周晓明江超杰王吉陈春尹来山
Owner UNIV OF ELECTRONICS SCI & TECH OF CHINA