Applications of diacylglycerol kinase gamma to treatment, diagnosis and predication of liver cancer
A diacylglycerol, kinase technology, applied in the diagnosis and prediction, the treatment of liver cancer
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Embodiment 1D
[0044] Example 1 Analysis of DGKγ expression
[0045] In order to explore the expression of DGKγ in liver cancer tissues, the real-time PCR method was used to detect the mRNA expression level of DGKγ gene in 79 pairs of liver cancer and paired paracancerous tissues and 17 cases of normal liver tissues. The result is as figure 1 As shown, the expression level of DGKγ in liver cancer tissues was significantly lower than that in paracancerous tissues and normal tissues (Pfigure 1 A). In addition, compared with para-cancerous tissues, the proportion of samples with low expression of DGKγ in liver cancer tissues was 50.6% (40 / 79), while the ratio of high expression of DGKγ was only 8.8% (7 / 79), suggesting that the DGKγ gene has an important role in liver cancer tissues. Significantly low expression in ( figure 1 B).
Embodiment 2
[0046] Example 2 Correlation between DGKγ expression level in liver cancer tissue and clinicopathological parameters and its possible impact on patient prognosis
[0047] In order to analyze the correlation between the expression level of DGKγ and the clinicopathological background of patients with liver cancer, the sex, age, tumor size and number, AFP, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin of 79 patients were collected and sorted out. Factors such as clinicopathological indicators. According to the relative expression level of DGKγ in patients, the patients were divided into low expression group and non-low expression group with cancer / paracancer <0.5 as the cut-off point. Analysis by Chi-square test or Fisher's exact test found that the expression of DGKγ was not correlated with patient gender, age, tumor size and number, AFP, ALT, AST, and Toatal bilirubin, but it was related to the background of HBV virus infection. relation....
Embodiment 3D
[0052] Example 3 Analysis of DGKγ Gene Transcription Regulatory Mechanism
[0053] In order to clarify the molecular mechanism of the low expression of DGKγ in liver cancer tissue, we first analyzed the deletion and mutation of DGKγ gene in liver cancer tissue. Through the analysis of the results of 25 cases of aCGH in our laboratory, no deletion of the DGKγ gene was found in the liver cancer tissues; by searching the cosmic database for DGKγ mutations, it was found that only 5 of the 942 liver cancer tissue samples had mutations, and the mutation rate was only 0.53%; therefore, deletion or mutation is not the main mechanism causing the low expression of DGKγ in liver cancer tissues.
[0054] Methylation of the promoter region is one of the important mechanisms for suppressing the expression of tumor suppressor genes. In order to determine whether the low expression of DGKγ in liver cancer tissues is related to the abnormal methylation of the DGKγ gene promoter region, we det...
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