Applications of diacylglycerol kinase gamma to treatment, diagnosis and predication of liver cancer

A diacylglycerol, kinase technology, applied in the diagnosis and prediction, the treatment of liver cancer

Active Publication Date: 2017-05-10
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Applications of diacylglycerol kinase gamma to treatment, diagnosis and predication of liver cancer
  • Applications of diacylglycerol kinase gamma to treatment, diagnosis and predication of liver cancer
  • Applications of diacylglycerol kinase gamma to treatment, diagnosis and predication of liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1D

[0044] Example 1 Analysis of DGKγ expression

[0045] In order to explore the expression of DGKγ in liver cancer tissues, the real-time PCR method was used to detect the mRNA expression level of DGKγ gene in 79 pairs of liver cancer and paired paracancerous tissues and 17 cases of normal liver tissues. The result is as figure 1 As shown, the expression level of DGKγ in liver cancer tissues was significantly lower than that in paracancerous tissues and normal tissues (Pfigure 1 A). In addition, compared with para-cancerous tissues, the proportion of samples with low expression of DGKγ in liver cancer tissues was 50.6% (40 / 79), while the ratio of high expression of DGKγ was only 8.8% (7 / 79), suggesting that the DGKγ gene has an important role in liver cancer tissues. Significantly low expression in ( figure 1 B).

Embodiment 2

[0046] Example 2 Correlation between DGKγ expression level in liver cancer tissue and clinicopathological parameters and its possible impact on patient prognosis

[0047] In order to analyze the correlation between the expression level of DGKγ and the clinicopathological background of patients with liver cancer, the sex, age, tumor size and number, AFP, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin of 79 patients were collected and sorted out. Factors such as clinicopathological indicators. According to the relative expression level of DGKγ in patients, the patients were divided into low expression group and non-low expression group with cancer / paracancer <0.5 as the cut-off point. Analysis by Chi-square test or Fisher's exact test found that the expression of DGKγ was not correlated with patient gender, age, tumor size and number, AFP, ALT, AST, and Toatal bilirubin, but it was related to the background of HBV virus infection. relation....

Embodiment 3D

[0052] Example 3 Analysis of DGKγ Gene Transcription Regulatory Mechanism

[0053] In order to clarify the molecular mechanism of the low expression of DGKγ in liver cancer tissue, we first analyzed the deletion and mutation of DGKγ gene in liver cancer tissue. Through the analysis of the results of 25 cases of aCGH in our laboratory, no deletion of the DGKγ gene was found in the liver cancer tissues; by searching the cosmic database for DGKγ mutations, it was found that only 5 of the 942 liver cancer tissue samples had mutations, and the mutation rate was only 0.53%; therefore, deletion or mutation is not the main mechanism causing the low expression of DGKγ in liver cancer tissues.

[0054] Methylation of the promoter region is one of the important mechanisms for suppressing the expression of tumor suppressor genes. In order to determine whether the low expression of DGKγ in liver cancer tissues is related to the abnormal methylation of the DGKγ gene promoter region, we det...

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Abstract

The invention provides applications of a diacylglycerol kinase gamma (DGK gamma) and an activator thereof to preparation of medicament for treating liver cancer, also provides applications of diacylglycerol kinase gamma and an agent for detecting diacylglycerol kinase gamma to preparation of an agent for diagnosis of liver cancer with bad prognosis, and also provides applications of diacylglycerol kinase gamma or the agent for detecting diacylglycerol kinase gamma to preparation of the agent for predicating prognosis conditions of liver cancer.

Description

technical field [0001] The invention relates to the technical field of treatment, diagnosis and prediction of liver cancer, in particular to the application of Diacylglycerol Kinase γ (DGKγ) in the treatment, diagnosis and prediction of liver cancer. Background technique [0002] Primary hepatocellular carcinoma (Hepatocellular carcinoma, HCC, referred to as liver cancer) is one of the most common malignant tumors in my country, ranking third in cancer incidence and second in mortality in China. There are about 740,000 new cases and 690,000 deaths of liver cancer in the world every year, and more than half of the cases occur in China. At present, surgery is still the most effective treatment for liver cancer. However, due to the hidden onset of liver cancer, many patients are diagnosed at an advanced stage and lose the opportunity for surgical treatment. For advanced liver cancer, the only existing targeted chemotherapy drug Sorafenib can only prolong the survival time of p...

Claims

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Application Information

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IPC IPC(8): A61K38/45A61K45/00A61P35/00
Inventor 鲁凤民贾俊巧姚明解王永峰康静婷郭正阳
Owner PEKING UNIV
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