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Rhein/chitosan hydrogel as well as preparation method and application thereof

A technology of rhein and chitosan solution is applied in pharmaceutical formulations, medical preparations with inactive ingredients, medical preparations containing active ingredients, etc. Easy injection, high mechanical strength effect

Active Publication Date: 2017-05-17
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved in the present invention is to overcome the deficiencies in the prior art, to provide a kind of injectable rhein / chitosan hydrogel with anti-inflammatory, antibacterial, anti-tumor properties, which can solve the problem of poor solubility of rhein, and Preserves the biological activity of rhein

Method used

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  • Rhein/chitosan hydrogel as well as preparation method and application thereof
  • Rhein/chitosan hydrogel as well as preparation method and application thereof
  • Rhein/chitosan hydrogel as well as preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0036] A kind of rhein / chitosan hydrogel of the present invention comprises rhein solution and chitosan solution, and rhein solution comprises rhein and NaHCO 3 The solution was prepared by the following method:

[0037] (1) Preparation of chitosan solution: Weigh 200 mg of chitosan in a centrifuge tube, add 10 mL of 1% acetic acid solution by volume, stir to dissolve, and configure a chitosan solution with a concentration of 20 mg / mL.

[0038] (2)NaHCO 3 Solution preparation: weigh 0.84g NaHCO 3 In a beaker, add ultrapure water and stir to dissolve, then transfer to a 100mL volumetric flask, add water to volume, and prepare NaHCO with a concentration of 0.1mol / L 3 solution.

[0039] (3) Weigh 2mg of rhein powder into the corresponding centrifuge tube, add 1mL of NaHCO from step (2) to the centrifuge tube while shaking 3 The solution obtained a rhein solution with a concentration of 2 mg / mL.

[0040] (4) Get 0.5mL of the chitosan solution of step (1) in a screw bottle, and ...

Embodiment 2

[0052] A kind of rhein / chitosan hydrogel of the present invention comprises rhein solution and chitosan solution, and rhein solution comprises rhein and NaHCO 3 The solution was prepared by the following method:

[0053] (1) NaHCO 3 Solution preparation: weigh 0.84gNaHCO 3 In a beaker, add ultrapure water and stir to dissolve, then transfer to a 100mL volumetric flask, add water to volume, and prepare NaHCO with a concentration of 0.1mol / L 3 solution.

[0054] (2) Preparation of rhein solution: Weigh 3 mg, 2.5 mg, 2 mg, and 1.7 mg of rhein in corresponding screw bottles, and add 1 mL of rhein with a concentration of 0.1 mol / L to each screw bottle while shaking. NaHCO 3 The solutions were prepared into rhein solutions of 3 mg / mL, 2.5 mg / mL, 2 mg / mL and 1.7 mg / mL respectively.

[0055] (3) Preparation of chitosan solution: Weigh 200 mg of chitosan in a centrifuge tube, add 10 mL of 1% acetic acid solution in volume ratio, ultrasonically dissolve, and configure a chitosan so...

Embodiment 3

[0065] A kind of application of rhein / chitosan hydrogel of the present invention in the treatment of microglia-mediated inflammatory disease, the specific method comprises the following steps:

[0066] 1. Preparation of drug base:

[0067] The configuration of drug-containing medium 1: take the rhein / chitosan hydrogel mother solution containing rhein at a concentration of 1.6 mg / mL, add it to the culture medium and dilute it to obtain drug-containing medium 1, and the rhein / chitosan hydrogel in drug-containing medium 1 The concentration of rhein contained in chitosan hydrogel was 4.5 μg / mL.

[0068] The configuration of drug-containing medium 2: take the mother liquor of rhein monomer with a concentration of 6 mg / mL, and add it to the culture medium to prepare drug-containing medium 2. The concentration of rhein monomer in drug-containing medium 2 is 4.5 μg / mL.

[0069] 2. Preparation of rat microglia (BV2 cell line) inflammatory model:

[0070] Rhein group: BV2 cells in the...

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Abstract

The invention discloses rhein / chitosan hydrogel as well as a preparation method and an application thereof. The rhein / chitosan hydrogel comprises a rhein solution and a chitosan solution, wherein the rhein solution comprises rhein and a NaHCO3 solution. The preparation method comprises the following steps: the rhein is dissolved in the NaHCO3 solution, and the rhein solution is obtained; the rhein solution is added to the chitosan solution with stirring under an ice bath condition, ultrasonic degassing is performed with the ultrasonic power being 70-120 W, then the solution is heated at the temperature of 32 DEG C or higher for 1-5 min, and the rhein / chitosan hydrogel is obtained. The rhein / chitosan hydrogel has the characteristics of being anti-inflammatory, antimicrobial, anti-tumor and the like, can solve the problems of poor solubility and short half-life of the rhein, retains biological activity of the rhein, is applied to drugs for treating microglial cell mediated inflammatory encephalopathy such as Alzheimer disease, Parkinson's disease, brain trauma, cerebral edema and the like, and has obvious treatment effect.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a rhein / chitosan hydrogel and a preparation method and application thereof. Background technique [0002] Rhein is a free anthraquinone substance derived from the traditional Chinese medicine rhubarb, and its molecular formula is CH 15 h 8 o 6 . It is a natural antioxidant and anti-inflammatory substance, with few toxic and side effects, and has good antioxidant and anti-inflammatory effects, so it has anti-tumor, anti-inflammatory, purgative, neuroprotective, lipid-lowering and other aspects. higher activity. [0003] Chitosan is the only alkaline polysaccharide that exists in large quantities in nature, and has low biological toxicity. Chitosan relies on its good biocompatibility (Biomacromolecules.2003.4: 1457-1465), special biological activities (anti-inflammatory, hemostatic, Tissue adhesion, lipid-lowering, cholesterol-lowering, blood-sugar-loweri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/192A61K31/722A61K9/06A61K47/36A61P25/28A61P25/16A61P25/00A61P29/00A61P7/10A61L27/52A61L27/54A61L27/20A61L26/00
CPCA61K9/06A61K31/192A61K31/722A61K47/36A61L26/0023A61L26/0066A61L26/008A61L27/20A61L27/52A61L27/54A61K2300/00C08L5/08
Inventor 王杨张翼郑俊郑飘唐涛范学工范荣
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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