Method for identifying rhizoma pinelliae and brooklet anemone root
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A technology of pinellia and tiger's palm, applied in the field of tiger's palm to identify pinellia, can solve the problems of unobvious characteristics of sub-tubers, difficult to identify, not rigorous enough, etc.
Active Publication Date: 2017-05-17
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology
In order to seek profit, there are many traders who processed tiger palms and then screened and polished them. The original sub-tubers have no obvious characteristics and are very similar to pinellia, making it difficult to identify them. If they are processed into decoction pieces, they can hardly be identified by their characteristics.
At present, there are still many problems in the identification of Pinellia and tiger palm. Traditionally, Pinellia is spherical or oval, and generally has no sub-t
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Embodiment 2
[0061] Example 2 Identification method of pinellia and tiger palm of the present invention
[0062] 1. Preparation of reference solution
[0063] Take an appropriate amount of Ophiopogon japonicus acid reference substance, add the mobile phase to dissolve it, and make a solution with a concentration of 0.05 mg / ml.
[0064] 2. Preparation of test solution
[0065] Take 1g of pinellia or tiger palm medicinal powder, add 20ml of water, ultrasonically extract for more than 30min, filter or centrifuge, take 10ml of clarified extract, add 0.1ml of phosphoric acid, extract 4 times with ethyl acetate, 20-30ml each time, and recover ethyl acetate Add 5ml of mobile phase to dissolve the ester and residue, and pass through a microporous membrane to get it.
[0066] 3. Chromatographic conditions
[0067] Mobile phase: A (acetonitrile)-B (0.1% phosphoric acid) (3:97)
[0068] Column: Agilent ZORBAX Eclipse Plus C 18 Liquid chromatography column (4.6×250mm, 5μm)
[0069] Flow rate: 0.8ml / min
[0070] De...
Embodiment 3
[0075] Example 3 Condition screening test of the extraction method of the present invention
[0076] 1. Investigation of extraction method
[0077] (1) The amount of extraction solvent
[0078] Weigh 1g of tiger palm sample, add 20ml, 30ml, 40ml of water respectively, and ultrasonically extract, respectively take 10ml of clear extract, add 0.1ml of acid, extract with ethyl acetate, recover solvent, dissolve with 5ml of mobile phase solution, and pass through microporous membrane , High performance liquid chromatography detection.
[0079] Table 3 Investigation of extraction solvent consumption
[0081] The peak area is the highest when the water volume is 20ml, and the extraction effect is the best.
[0082] (2) Investigation of extraction time
[0083] Weigh 1g of tiger palm sample, add 20ml of water, and ultrasonically extract. The extraction time is 30min, 45min, 60min...
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Abstract
The invention provides use of triglochinic acid in identification of medicinal materials, i.e., rhizoma pinelliae and brooklet anemone root. The invention provides a method for identifying the rhizoma pinelliae and the brooklet anemone root. The method comprises the following steps: a. taking the medicinal materials to be detected; b. taking a reference substance, i.e., the triglochinic acid; c. detecting; d. carrying out result judgment, wherein the medicinal material containing the triglochinic acid is the brooklet anemone root, and the medicinal material which does not contain the triglochinic acid is the rhizoma pinelliae. The method can be used for distinguishing whether the rhizoma pinelliae is adulterated with the brooklet anemone root or not by identifying the presence or absence of the triglochinic acid; if containing the triglochinic acid, the rhizoma pinelliae is adulterated with the brooklet anemone root. The identification process is simple, fast and effective, and the method can be used for identifying the brooklet anemone root and distinguishing whether the rhizoma pinelliae is adulterated with the brooklet anemone root or not; after being processed, the brooklet anemone root still can be identified; the method is stable and reliable.
Description
Technical field [0001] The invention relates to a method for identifying pinellia and tiger palm. Background technique [0002] Pinellia is the dry tuber of Pinelliaternata (Thunb.) Breit, a plant of Pinellia genus in the Araceae family. Tiger palm is a dry tuber of Pinelliae Pedatisecta Schott, a plant belonging to the genus Pinelliae of the Araceae family. [0003] There are many counterfeit products of Pinellia ternata, mainly including tiger palm, Araceae, Pinellia ternata, etc. After processing, the appearance of these medicinal materials is very similar to Pinellia pinellia, which is difficult to identify. Unscrupulous vendors often add this to Pinellia Several medicinal materials were illegally sought for profits, which seriously harmed the interests of Pinellia ternata growers and Pinellia decoction pieces production enterprises. At the same time, it also seriously affected the quality, efficacy and medication safety of Pinellia medicinal materials. [0004] At present, tig...
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IPC IPC(8): G01N30/02G01N30/90
CPCG01N30/02G01N30/90
Inventor 李敏陈辉敬勇陶玲何金晓刘佳灵
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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