Phalaenopsis medium and preparation method thereof
A medium and camphor technology, applied in the field of plant tissue culture, can solve the problems of inability to use the seeding method for sexual reproduction, low germination rate, etc., and achieve the goal of improving the survival rate of cultivation, prolonging the fertilization period, and ensuring continuity and uniformity. Effect
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Embodiment 1
[0020] 1) Raw material treatment: semi-carbonize 50kg of straw, crush 20kg of vermiculite, 30kg of perlite, 15kg of weathered coal, 20kg of plant ash and 50kg of peat into particles with a particle size of 2mm;
[0021] 2) Mixing: mix the raw materials prepared in step 1), 40 kg of silk reeling wastewater and 45 kg of fermented poultry and livestock manure, and stir evenly to obtain a culture medium mixture;
[0022] 3) Spraying: Mix 15kg of sucrose with water at a ratio of 1:100, and spray it on the medium mixture, so that the medium mixture and the plant growth regulator are fully mixed;
[0023] 4) Disinfection: Disinfect the culture medium prepared in step 3), and then dry it;
[0024] 5) Place the culture medium in an environment with a temperature of 28°C, a light intensity of 3000 lux, and a humidity of 70% to plant and cultivate Cinnamomum camphora.
Embodiment 2
[0026] 1) Raw material treatment: semi-carbonize 80kg of straw, crush 35kg of vermiculite, 50kg of perlite, 20kg of tea waste residue, 25kg of weathered coal, 30kg of plant ash and 70kg of peat into particles with a particle size of 10mm;
[0027] 2) Mixing: Mix the raw materials prepared in step 1), 60 kg of silk reeling wastewater and 60 kg of fermented poultry and livestock manure, and stir evenly to obtain a culture medium mixture;
[0028] 3) Spraying: Mix 30kg of sucrose with water at a ratio of 1:100, and spray it on the medium mixture, so that the medium mixture and the plant growth regulator are fully mixed;
[0029] 4) Disinfection: Disinfect the culture medium prepared in step 3), and then dry it;
[0030] 5) Place the culture medium in an environment with a temperature of 25°C, a light intensity of 1500 lux, and a humidity of 60% to plant and cultivate Cinnamomum camphora.
Embodiment 3
[0032] 1) Raw material treatment: semi-carbonize 60kg of straw, crush 30kg of vermiculite, 40kg of perlite, 45kg of tea waste residue, 20kg of weathered coal, 25kg of plant ash and 60kg of peat into particles with a particle size of 5mm;
[0033] 2) Mixing: Mix the raw materials prepared in step 1), 50 kg of silk reeling wastewater and 50 kg of fermented poultry and livestock manure, and stir evenly to obtain a culture medium mixture;
[0034] 3) Spraying: Mix 25kg of sucrose with water at a ratio of 1:100, and spray it on the medium mixture, so that the medium mixture and the plant growth regulator are fully mixed;
[0035] 4) Disinfection: Disinfect the culture medium prepared in step 3), and then dry it;
[0036] 5) Place the culture medium in an environment with a temperature of 30°C, a light intensity of 5000 lux, and a humidity of 80% to plant and cultivate Cinnamomum camphora.
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