Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing recombinant glycoprotein with modified glycosylation

A glycoprotein and glycosylation technology, applied in biochemical equipment and methods, glycosylase, chemical instruments and methods, etc., can solve the problem of high cost of recombinant protein

Active Publication Date: 2021-03-16
ONENESS BIOTECH
View PDF12 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, currently available methods for glycosylation remodeling often require multiple enzymes and / or multiple steps, resulting in high cost of manufacturing glycoengineered recombinant proteins

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing recombinant glycoprotein with modified glycosylation
  • Method for producing recombinant glycoprotein with modified glycosylation
  • Method for producing recombinant glycoprotein with modified glycosylation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0087] method

[0088] i. Construction of expression vectors for the production of fucosidase or endoglycosidase

[0089] To construct an expression vector for fucosidase or endoglycosidase, the fucosidase or endoglycosidase gene is isolated by conventional techniques and codon-optimized based on codon usage of hamster cells. The synthetic gene was prepared by GeneArt Corp, and inserted into pcDNA3.1B(-)Myc-His vector (Invitrogen, US) at the restriction enzyme site Bgl II / EcoR I ( figure 2 middle A). The expression cassette for alpha-fucosidase, from 5' to 3', contains the Kozak sequence, the Igk leader sequence, the fucosidase coding sequence and the His-tag coding sequence. figure 2 middle B. The endoglycosidase expression cassette, from 5' to 3', contains the Kozak sequence, the Igk leader sequence, the endoglycosidase coding sequence, and the His-tag coding sequence. figure 2 middle B.

[0090] ii. Preparation of defucosylated antibodies

[0091] Antibody-producin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Genetically engineered host animal cells are capable of producing glycoproteins with modified glycosylation patterns, such as defucosylation and / or monoglycosylation. Such host animal cells can be engineered to express fucosidases, endoglycosidases, or both.

Description

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS [0002] This application claims the benefit of US Provisional Application No. 61 / 954,337, filed March 17, 2014, the entire contents of which are incorporated herein by reference. Background technique [0003] Glycosylation is essential for the structure and function of glycoproteins. For example, glycosylation is believed to affect protein folding (and thus stability) and / or the biological activity of glycoproteins. Demand for therapeutic recombinant glycoproteins, especially monoclonal antibodies, has grown strongly in the last two decades. Previous studies have shown that small differences in the structure of recombinant glycoproteins may affect the bioactivity and pharmacokinetics of glycoproteins. For example, darbepoetin alfa is a highly glycosylated analog of recombinant human erythropoietin (EPO) with two additional N-linked glycosylation sites. Compared with endogenous or recombinant EPO, additional glycosylation incre...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/10C12N15/56C12N15/85C12P21/00
CPCC07K16/2887C07K16/4291C12P21/005C12N9/2405C07K2317/14C07K2317/41C12Y302/01096C12Y302/01051C12N9/2402C07K16/00C12N15/85Y02P20/52
Inventor 陈念宜吴哲豪陈虹琦汤竣钧
Owner ONENESS BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com