Podocarpus macrophyllus culture medium and preparation method thereof
A culture medium and Podocarpus technology, applied in the field of plant tissue culture, can solve the problems of not being suitable for most plants, low use efficiency, slow growth, etc., and achieve the effects of improving the survival rate of cultivation, reasonable proportioning, and balanced nutrition
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Embodiment 1
[0019] 1) Raw material processing: semi-carbonize 30kg of bagasse and 60kg of straw, crush 25kg of vermiculite, 55kg of weathered coal, 20kg of plant ash and 90kg of peat into particles with a particle size of 2mm;
[0020] 2) Mixing: Mix the raw materials prepared in step 1), 30 kg of fermented poultry manure and 10 kg of superphosphate, and stir evenly to prepare a medium mixture;
[0021] 3) Spraying: Mix 2kg of plant growth regulator with water at a ratio of 1:100, and spray it on the medium mixture, so that the medium mixture and plant growth regulator are fully mixed;
[0022] 4) Disinfection: Disinfect the culture medium prepared in step 3), and then dry it;
[0023] 5) Place the medium in an environment with a temperature of 25°C, a light intensity of 1500 lux, and a humidity of 60% to plant and cultivate Podocarpus pine.
Embodiment 2
[0025] 1) Raw material treatment: Semi-carbonize 50kg of bagasse and 80kg of straw, crush 40kg of vermiculite, 75kg of weathered coal, 25kg of tea waste residue, 45kg of plant ash and 110kg of peat into particles with a particle size of 15mm;
[0026] 2) Mixing: Mix the raw materials prepared in step 1), 50 kg of fermented poultry manure and 15 kg of superphosphate, and stir evenly to obtain a medium mixture;
[0027] 3) Spraying: Mix 5 kg of plant growth regulator with water at a ratio of 1:100, and spray it on the medium mixture, so that the medium mixture and plant growth regulator are fully mixed;
[0028] 4) Disinfection: Disinfect the culture medium prepared in step 3), and then dry it;
[0029] 5) Place the medium in an environment with a temperature of 30°C, a light intensity of 5000 lux, and a humidity of 80% to plant and cultivate Podocarpus pine.
Embodiment 3
[0031] 1) Raw material treatment: semi-carbonize 40kg of bagasse and 70kg of straw, crush 30kg of vermiculite, 60kg of weathered coal, 40kg of tea waste residue, 30kg of plant ash and 100kg of peat into particles with a particle size of 10mm;
[0032] 2) Mixing: Mix the raw materials prepared in step 1), 40 kg of fermented poultry manure and 13 kg of superphosphate, and stir evenly to obtain a medium mixture;
[0033] 3) Spraying: Mix 4 kg of plant growth regulator with water at a ratio of 1:100, and spray it on the medium mixture, so that the medium mixture and plant growth regulator are fully mixed;
[0034] 4) Disinfection: Disinfect the culture medium prepared in step 3), and then dry it;
[0035] 5) Place the medium in an environment with a temperature of 28°C, a light intensity of 3000 lux, and a humidity of 70% to plant and cultivate Podocarpus pine.
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