The invention provides a preparation method of a bionic mineralized
collagen scaffold. The preparation method comprises the following steps: 1), preparing a bionic mineralized liquid: adjusting the pHvalue of
carboxymethyl chitosan-
amorphous calcium phosphate (or amorphous
strontium phosphate or amorphous
strontium carbonate)
nanocomposite liquid to be lower than an
isoelectric point of CMC; 2),preparing the bionic mineralized
collagen scaffold: mixing the bionic mineralized liquid and acid-dissolved type-I collagen, then putting into a
dialysis bag, sealing, then putting into a PBS buffer liquid, self-assembling and mineralizing for 2-5 days, then adding an alkaline CMC / ACP (ASP or ASC) liquid into the
dialysis bag, and changing the PBS buffer liquid; 1-3 days later, putting the
dialysis bag into deionized water for dialyzing for 10-72h, changing the deionized water for several times, centrifuging the liquid in the bag to obtain
collagen gel, and performing
freeze drying to obtain the bionic mineralized
collagen scaffold. By the preparation method, collagen self-
assembly and mineralization are cooperatively performed, intra-
collagen fiber mineralization is achieved at relativelyhigh efficiency, and osteogenic ability-promoting
strontium is integrated into the collagen
scaffold, so that the preparation method has a wide clinical application prospect.