Pressurizing/depressurizing dual-purpose operating console type chromatographic column

Abstract

The invention discloses a pressurizing / depressurizing dual-purpose operating console type chromatographic column. The pressurizing / depressurizing dual-purpose operating console type chromatographic column comprises an experiment rack, a test tube clamp, a screw rod, a nut, a stepping motor, a chromatographic column, a liquid inlet funnel, a liquid storage ball, a double-hole polished column cover, an annular stirring rod, a connection bar, a gas inlet / outlet pipeline, a positive / negative pressure dual-purpose vacuum pump, connection wires and an operating console, wherein in the chromatographic column, the experiment rack is provided with the test tube clamp, and the upper end is connected with the screw rod; the screw rod is sleeved with the nut, and the upper end is connected with the stepping motor; the chromatographic column is fixed on the experiment rack by the test tube clamp, and the upper end is connected with the lower end of the liquid storage ball; the liquid inlet funnel is connected with the liquid storage ball; the double-hole polished column is positioned at the top end of the chromatographic column, and the annular stirring rod and the gas inlet / outlet pipeline are connected with the connection bar and the positive / negative pressure dual-purpose vacuum pump through a hole respectively; and the stepping motor and the positive / negative pressure dual-purpose vacuum pump are connected to the operating console via the wires. The chromatographic column disclosed by the invention adopts an operating console type, and is simple to operate; and the positive / negative pressure dual-purpose vacuum pump can discharge bubbles among stationary phases through depressurizing and can also enable eluting agent to smoothly pass through a silica gel column through pressurizing.

Description

technical field [0001] The invention relates to a purification and separation chromatography device used in the fields of biotechnology, pharmacy, food, chemical industry, etc., in particular to a dual-purpose operating bench-type chromatography column for both pressure and pressure. Background technique [0002] Column chromatography is a separation and purification method commonly used in chemical laboratories. According to the different distribution coefficients of the components in the sample mixture in the stationary phase (filler) and mobile phase (eluent), the components are separated after repeated distributions. A method of separation. The columns used in column chromatography are generally ordinary glass tubes or plexiglass tubes, and the stationary phase and mobile phase should be selected according to different separation objects. The particle size of the stationary phase has a great influence on the separation effect of the target compound. The smaller the part...

AI Technical Summary

Problems solved by technology

However, when configuring the stationary phase of column chromatography, bubbles are likely to be generated, which makes the stationary phase uneven and affects the separation effect when eluting the sample.

Images