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A kind of small peptide and its application

A product, anti-oxidation technology, applied in the biological field, to achieve the effect of alleviating diabetic complications and strong anti-oxidation ability

Active Publication Date: 2020-01-07
陕西乾翔健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is a lack of drugs and functional foods that can regulate the abnormal proliferation of VSMCs induced by high glucose, regulate the apoptosis of smooth muscle cells in a high glucose environment, and make it tend to a normal state, so as to alleviate diabetic complications and delay or prevent the development of AS

Method used

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  • A kind of small peptide and its application
  • A kind of small peptide and its application
  • A kind of small peptide and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The antioxidant activity of embodiment 1 small peptide of the present invention

[0022] This example investigates the antioxidant activity of the small peptides of the present invention.

[0023] 1. Determination of Oxygen Radical Absorption Capacity (ORAC)

[0024] Pipette 20 μL of the sample solution to be tested (the final concentration in the reaction system is 50 μM) and 80 μL sodium fluorescein solution (the final concentration of fluorescein sodium in the reaction system is 175 nM) into each well of a black 96 microwell plate, mix well, and After incubation at 37°C for 15 minutes. The reaction was started by adding 100 μL of AAPH (azobisisobutylamidine hydrochloride, the final concentration of AAPH in the reaction system was 24 mM) to each well of the microplate with a multichannel pipette. Then the microplate was placed in a microplate reader, shaken and mixed, and measured at 37°C, excitation wavelength of 485nm, and emission wavelength of 538nm. The fluore...

Embodiment 2

[0032] Example 2 The protective effect of the small peptide of the present invention on the abnormal proliferation of VSMCs induced by high glucose

[0033] The following experiments were carried out using the small peptide of the present invention.

[0034] 1. Detection of cell proliferation by thiazolium blue (MTT) method

[0035] Take VSMCs in the logarithmic growth phase, and adjust the cell suspension to 5×10 with low-sugar DMEM medium containing 2% FBS 4 cells / mL, inoculated in 96-well plates, 100 μL per well (100 μL low-sugar DMEM medium containing 2% FBS was added to the blank control group), and the number of cells was 5×10 3 pcs / well, placed at 37°C, 5% CO 2 After culturing in the incubator for 48 h, the culture medium was aspirated, and 100 μL of low-sugar DMEM medium was added to each well. The blank control group did not add any substances, and the other wells were randomly divided into three groups: the normal group did not add any substances; the high-sugar g...

Embodiment 3

[0048] Example 3 Effect of small peptides of the present invention on apoptosis of VSMCs incubated with high sugar

[0049] The following experiments were carried out using the small peptide of the present invention.

[0050] Annexin V-FITC / PI double staining method to detect cell apoptosis rate: the apoptosis rate of cells in each group was detected by Annexin V-FITC cell apoptosis detection kit (Beiyuntian Bioreagent Company), that is, Annexin V-FITC / PI double staining Law. VSMCs in the logarithmic growth phase were digested with 0.05% EDTA-trypsin solution, and the cell suspension was adjusted to 5×10 with low-sugar DMEM medium containing 10% FBS. 4 cells / mL, seeded in 6-well plate, 2 mL per well, so that the number of cells was 1×10 5 pcs / well, placed at 37°C, 5% CO 2 After culturing in the incubator for 48 hours, the culture solution was sucked off, replaced with serum-free low-sugar DMEM medium, and randomly divided into three groups: the normal group did not add any ...

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Abstract

The invention provides a small peptide and application thereof, and relates to the technical field of biology. The amino acid sequence of the small peptide is shown as SEQ ID NO:1. The invention also provides the application of the small peptide to preparation of functional food for alleviating diabetic complications, anti-atherosclerosis functional food, antioxidant functional food, a free radical scavenging product and a lipid peroxidation inhibition product. The small peptide not only has relatively high antioxidant capability, but also can be used for regulating high glucose-induced abnormal proliferation of VSMCs (vascular smooth muscle cells) and regulating apoptosis of the VSMCs in a high glucose environment to finally make the VSMCs tending to get normal, and thus has a broad development prospect on the aspects of prevention of atherosclerosis formation in the high glucose environment, alleviation of other diabetic complications and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a small peptide and its application. Background technique [0002] Diabetes mellitus is a group of metabolic diseases characterized by chronic elevated blood glucose levels. Auto-oxidation of glucose, enhanced protein glycosylation and abnormal lipid metabolism caused by hyperglycemia are the main reasons for the increase of free radicals in diabetic patients, especially the increase of reactive oxygen species (ROS), and also lead to a significant decrease in the activity of some antioxidant enzymes. Therefore, diabetic patients are in a state of oxidative stress to a certain extent. Oxidative stress not only participates in the occurrence, development and deterioration of diabetes, but also participates in the important pathological changes of diabetic complications - atherosclerosis (AS) in multiple organs. Vascular smooth muscle cells (VSMCs), an important component of the vesse...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06A61K38/08A61P39/06A61P3/10
CPCA61K38/00C07K7/06
Inventor 沈新春曹小舟陈思远俞凌汤晓智方勇
Owner 陕西乾翔健康科技有限公司
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