Microfluidics cell drug concentration gradient generator

Abstract

The invention discloses a microfluidics cell drug concentration gradient generator. The microfluidics cell drug concentration gradient generator comprises four groups of liquid inlet holes which are communicated through microchannels so as to form drug and culture medium laminar flow combinations of different volume proportions in the microchannels; one group of microchannels with micro-mixing function can ensure that drug and culture medium laminar flow combinations of different volume proportions can be fully mixed to finally form different concentrations which can form nine concentration gradients together with liquid concentrations in two channels led out from a liquid inlet directly; one group of chambers are connected with the micro-mixed channels for cell culturing and drug interaction research, and nine chambers are independent from one another. The concentration gradient generator is hardly influenced by liquid inlet rate during the generation of concentration gradients, good linear concentration gradients can be formed, and the concentration gradient generator has a simple structure and provides a brandnew platform for screening and detection of cell drugs.

Description

technical field [0001] The invention relates to the field of designing microfluidic chips, in particular to a drug concentration gradient generator for cells. Background technique [0002] Drug screening, as the initial process and key step of new drug research, is to screen out high-efficiency new drugs or lead compounds from natural or synthetic compounds, and evaluate the biological activity, pharmacological effect and medicinal value of substances that may be used as drugs [1]. With the rapid development of cell biology technology, its microquantification and ultra-high-throughput advantages provide a way for high-throughput new drug screening based on the cell level [2]. High-throughput screening based on cell models can monitor and quantitatively analyze a variety of cellular events at the population cell level or single cell level[3] through a single experiment, providing reliable information for the initial screening of drugs. Traditional high-throughput screening ...

AI Technical Summary

Problems solved by technology

Because the number of concentrations = the number of layers + 2, if you want to get different concentrations, you will need a lot of layers, which will lead to lengthy chip channels and complex structures

This makes its scalability poor and it is difficult to achieve high-throughput biological analysis

In addition, the flow rate has a great influence on the stability of the concentration gradient (a good gradient can be formed when the flow rate is 0.001m / s-0.01m / s, and when the flow rate is greater than 0.01m / s, the gradient changes significantly), which is only suitable for ultra-low speed. cell analysis

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