siRNA (small interfering ribonucleic acid) of long noncoding RNA (ribonucleic acid) PCGEM1 (prostate cancer gene expression marker 1) related to ovarian cancer and endometrial cancer and application

An endometrial cancer, long non-coding technology, applied in the siRNA and application field of long non-coding RNA PCGEM1, can solve the problem of lack of lncRNA PCGEM1 and other problems

Active Publication Date: 2017-06-06
THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the expression of lncRNA PCGEM1 in ovarian cancer and endometrial cancer tissues, and finding effective early onset biomarkers and related molecular mechanisms can guide the clinical diagnosis and treatment of ovarian cancer and endometrial cancer and treatment are important

Method used

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  • siRNA (small interfering ribonucleic acid) of long noncoding RNA (ribonucleic acid) PCGEM1 (prostate cancer gene expression marker 1) related to ovarian cancer and endometrial cancer and application
  • siRNA (small interfering ribonucleic acid) of long noncoding RNA (ribonucleic acid) PCGEM1 (prostate cancer gene expression marker 1) related to ovarian cancer and endometrial cancer and application
  • siRNA (small interfering ribonucleic acid) of long noncoding RNA (ribonucleic acid) PCGEM1 (prostate cancer gene expression marker 1) related to ovarian cancer and endometrial cancer and application

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Experimental program
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Embodiment 1

[0021] 1. The expression of Lnc RNA PCGEM1 in ovarian cancer patients and normal ovarian tissue, endometrial cancer and normal endometrial tissue.

[0022] 1. Specimen collection.

[0023] With the patient’s knowledge, ovarian cancer and normal ovarian tissue, endometrial cancer and normal endometrial tissue samples were collected during the operation, washed with normal saline, and stored in liquid nitrogen or -80°C ultra-low temperature refrigerator for later use; The specimens were collected by Dr. Chen in the operating room of the First Affiliated Hospital of China Medical University from June 2014 to June 2015.

[0024] 2. Primer design.

[0025] According to the location information, the partial sequence information of the long non-coding RNAEUlncRNA1 was found in UCSC (Mar.2006) for chr1, and the primer PCR primers of PCGEM1 were designed using Primer Premier5.0 according to the obtained sequence information.

[0026] Primers used for PCR detection.

[0027] Upstream...

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Abstract

The invention belongs to the field of oncomolecularbiology and particularly relates to siRNA (small interfering ribonucleic acid) of long noncoding RNA (ribonucleic acid) PCGEM1 (prostate cancer gene expression marker 1) related to an ovarian cancer and an endometrial cancer, and an application. Nucleotide sequences of sense and antisense of the siRNA are GGAAACAACCUUUAACUUA and UAAGUUAAAGGUUGUUUCC respectively. The long noncoding RNA is related to propagation and apoptosis ability of cancer cells, and can serve as a diagnosis marker of the ovarian cancer and the endometrial cancer; a molecular target is provided for the treatment of the ovarian cancer and the endometrial cancer; the siRNA designed according to the long noncoding RNA can be applied to preparation of antitumor drugs.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and in particular relates to siRNA and application of long-chain non-coding RNA PCGEM1 related to ovarian cancer and endometrial cancer. Background technique [0002] Ovarian cancer and endometrial cancer are the deadliest gynecological malignancies with a high incidence rate and an increasing trend in recent years, seriously threatening the life and health of women in my country. Early screening and early diagnosis and treatment are crucial to the prognosis and efficacy of patients with ovarian cancer and endometrial cancer. [0003] In recent years, the role of long noncoding RNA (long noncoding RNA, lncRNA) in the occurrence, development, invasion, metastasis, and drug resistance of tumors has become a research hotspot. Long non-coding RNA is a type of non-coding RNA that is located in the nucleus or cytoplasm, with a transcript length greater than 200 nt, and regulates the expression l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K48/00A61K31/713A61P35/00
CPCC12N15/113C12N2310/14C12N2310/113
Inventor 赵杨宗志红陈说刘瑶
Owner THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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