A kind of bacillus subtilis tolerant to limulusin and preparation method thereof

A technology of Bacillus subtilis and lysate, which is applied in the field of Bacillus subtilis and its preparation, can solve the problems of not being able to overcome the degradation effect of lysine I to inhibit and kill lysate I, and achieve the effect of strong tolerance
CN106834202BActive Publication Date: 2020-08-04SHENZHEN POLYTECHNIC
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHENZHEN POLYTECHNIC
Publication Date
2020-08-04

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Abstract

A limulusin-tolerant Bacillus subtilis and a preparation method thereof of the present invention, comprising the steps of: culturing Bacillus subtilis species in a culture medium to obtain Bacillus subtilis; adding limulusin to a new culture medium to prepare Different mediums with different concentrations of lysate; first inoculate the obtained Bacillus subtilis in the medium with lower lysate concentration for streak culture to obtain Bacillus subtilis tolerant to a lower concentration of lysate; then in the next lysate Insert the obtained Bacillus subtilis tolerant to lower concentration of TAL into the culture medium with higher concentration for streak culture, and so on, gradually increase the concentration of TAL, and cultivate Bacillus subtilis that can tolerate higher concentration TAL . In the invention, the culture medium added with gradient concentration of limulus is continuously used for long-term directional breeding of bacillus subtilis, and the anti-limulusin resistant strain is obtained. The strains obtained by the long-term continuous induction of limulus have a strong tolerance to limulus.
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Description

technical field

[0001] The invention relates to the field of microbes, in particular to a limulusin-tolerant bacillus subtilis and a preparation method thereof. Background technique

[0002] Limulusin is a cationic antibacterial peptide isolated from small granules of Limulus blood cells. It has biological activities such as antibacterial, antifungal, antiviral, inhibiting tumor cell proliferation and inducing cancer cell differentiation. It is an antibacterial peptide with great potential application value. peptide. Limulusin mainly has three production methods: separation and purification from Limulus blood cells, chemical synthesis and genetic engineering expression. Due to the scarcity of horseshoe crab resources and the high cost of chemical synthesis, the first two methods are difficult to carry out large-scale production. Therefore, the production of horseshoe crab by genetic engineering expression is the only way to realize its industrialization.

[0003] The resea...

Claims

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