A Method for Evaluating Eye Irritation Using Mouse Kidney Cell System

Abstract

The invention discloses a method for evaluating eye irritation by means of a mouse kidney cell system, and the method is applicable to evaluation of cosmetic raw material irritation, in particular to evaluation of eye irritation of surfactants. According to the method, when irritation of a test material to cells exists, the cells contract, fully-fused cells are damaged, and when fluorescein acts on damaged cell membranes, the damage degree of the cell membranes is indirectly reflected according to the amount of leaked fluorescein, so that irritation of the test material is represented. The test material used by the method is stable in population, reliable in source and high in fusing speed, a cell growth culturing membrane is easy to form, and a guarantee is provided for stability of a test; and when the mouse kidney cell system is used for evaluation of irritation damage of the surfactants, a result is in good correlation with a result of a rabbit eye animal test.

Description

technical field

[0001] The invention relates to a method for evaluating eye irritation by using mouse kidney cells, which is suitable for evaluating the irritation of cosmetic raw materials, especially for evaluating the eye irritation of surfactants. Background technique

[0002] Surfactant is an important fine chemical product, which is widely used at home and abroad. It is known as "industrial monosodium glutamate", which can significantly reduce the surface tension of the solution. Surfactants are widely used in many industrial fields such as cosmetic preparation, food processing, pesticide and pharmaceutical processing, textile printing and dyeing, and detergent production. When surfactants are used in the production of cosmetics or detergents, the probability of contact with consumers' eyes is greatly increased, and they are often irritating or even corrosive to consumers' eyes.

[0003] Rabbit eye irritation test (Draize test) is a classic method to detect eye irrita...

Examples

Embodiment 1

[0057] 1. Experimental materials

[0058] 1. Experimental reagent grade equipment

[0059] Fetal bovine serum (Gibco company), the culture medium used in the embodiment all refers to the DMEM culture medium (Gibco company) without phenol red low sugar containing glutamine, fluorescein sodium (Shanghai Aladdin Biochemical Technology Co., Ltd.), insert cell Petri dish (Corning Millicell insert cell culture dish, 24 wells), collagenase I (Gibco company), sodium chloride, potassium chloride, anhydrous potassium hydrogen phosphate, anhydrous disodium hydrogen phosphate, glucose, deionized Water, fluorescence spectrophotometer (Shanghai Yidian-Analytical Instrument General Factory, 970CRT);

[0060] 2. Test substance

[0061] According to the OECD database (reference: Eye irritation. Reference chemicals databank (Second edition) [1998]), select 5 types of surfactants with known eye irritation ratings, see Table 1 for details.

[0062] Table 1 List of test substances

[0063] ...

technical field

[0001] The invention relates to a method for evaluating eye irritation by using mouse kidney cells, which is suitable for evaluating the irritation of cosmetic raw materials, especially for evaluating the eye irritation of surfactants. Background technique

[0002] Surfactant is an important fine chemical product, which is widely used at home and abroad. It is known as "industrial monosodium glutamate", which can significantly reduce the surface tension of the solution. Surfactants are widely used in many industrial fields such as cosmetic preparation, food processing, pesticide and pharmaceutical processing, textile printing and dyeing, and detergent production. When surfactants are used in the production of cosmetics or detergents, the probability of contact with consumers' eyes is greatly increased, and they are often irritating or even corrosive to consumers' eyes.

[0003] Rabbit eye irritation test (Draize test) is a classic method to detect eye irrita...

Examples

Embodiment 1

[0057] 1. Experimental materials

[0058] 1. Experimental reagent grade equipment

[0059] Fetal bovine serum (Gibco company), the culture medium used in the embodiment all refers to the DMEM culture medium (Gibco company) without phenol red low sugar containing glutamine, fluorescein sodium (Shanghai Aladdin Biochemical Technology Co., Ltd.), insert cell Petri dish (Corning Millicell insert cell culture dish, 24 wells), collagenase I (Gibco company), sodium chloride, potassium chloride, anhydrous potassium hydrogen phosphate, anhydrous disodium hydrogen phosphate, glucose, deionized Water, fluorescence spectrophotometer (Shanghai Yidian-Analytical Instrument General Factory, 970CRT);

[0060] 2. Test substance

[0061] According to the OECD database (reference: Eye irritation. Reference chemicals databank (Second edition) [1998]), select 5 types of surfactants with known eye irritation ratings, see Table 1 for details.

[0062] Table 1 List of test substances

[0063] ...

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